Terpenoids from Fructus Leonuri and Their Anti-Inflammatory Activities
FAN Yun-qiu1,2, PENG Cheng1,2, XIONG Liang1,2, ZHOU Qin-mei1,2,3, PENG Fang1,2, HE Yu-lin1,2, AO Hui3*, DAI Ou1,2*
1. State Key Laboratory of Southwestern Chinese Medicine Resources, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;
2. Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;
3. Institute of Traditional Chinese Medicine Innovation, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
OBJECTIVE To investigate the chemical constituents of Fructus Leonuri and their anti-inflammatory activities. METHODS The chemical constituents were isolated and purified by column chromatography over macroporous adsorption resin and silica gel, as well as semi-preparative HPLC. Their structures were identified by physicochemical properties and NMR and MS data. The anti-inflammatory activities of the compounds were evaluated by measuring their inhibitory effects on nitric oxide (NO) release in LPS induced RAW 264.7 macrophages. RESULTS Four terpenoids were isolated from the n-butanol extract of Fructus Leonuri, including a new compound. Their structures were identified as (-)-(5R, 6S, 8R)-5, 6-dihydroxy-dihydroactinidiolide (1), citroside A (2), icariside C3 (3), and leojaponin K (4). CONCLUSION Compound 1 is a new terpenoid. Compound 3 is isolated from the genus Leonurus for the first time. Compounds 2 and 4 are isolated from Fructus Leonuri for the first time. Among them, compound 4 cound inhibit NO release significantly in LPS-induced RAW264.7 cells, suggesting that it has a potential anti-inflammatory effect.
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2023, Vol. 58 
