Changes of Caffeoylquinic Acids in Crude and Processed Lonicera japonica
ZHANG Xue1,2, GUO Dong-xiao2, CUI Wei-liang2, LUAN Yong-fu2, SUN Qian1, ZHANG De-xin1, ZHAO Shu-xiu1, LIN Yong-qiang2*, LIU Qing-zhi1*
1. Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 2. Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM, Shandong Institute for Food and Drug Control, Jinan 250101, China
Abstract：OBJECTIVE To establish a method for determination of caffeoylquinic acids in processed products of Lonicera japonica, and to study the content changes of caffeoylquinic acids in crude and processed L. japonica. METHODS The chromatographic conditions such as extraction method, detection wavelength, and mobile phase composition were determined, and the specificity, linearity, durability, repeatability, recovery, and precision of the established analytical method were investigated. RESULTS The extraction solvent was 50% methanol, the solid-liquid ratio was 1∶200, and ultrasonic extraction was carried out for 30 minutes. The content was determined by HPLC, the column was ostade-cylsilane column(250 mm×4.6 mm, 5 μm), the mobile phase was acetonitrile(A)-0.4% phosphoric acid solution(B) by gradient elution, the detection wavelength was set at 327 nm. The specificity, linearity, durability, repeatability, recovery, and precision of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid meet corresponding requirements. CONCLUSION The method is simple, and can be used for simultaneous determination of 6 phenolic acids in processed products of L. japonica Furthermore. It is found that the contents and ratio of 6 caffeoylquinic acids changed significantly before and after processed, which provides a basis for the study of processing technology, quality control, and activity researches of L. japonica.
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2022, Vol. 57 
