Effect of miR-204-5p on SH-SY5Y cells Injury Induced by Oxygen-glucose Deprivation and Reoxygenation
TU Yu-jun1,2, XIANG Pu3, GUO Wen-jia1,2, GU Chao1,2, TAN Xiao-dan1,2, YANG Jun-qing1,2*
1. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 400016, China; 2. Department of Pharmacology, Chongqing Medical University, Chongqing 400016, China; 3. Dianjiang People's Hospital of Chongqing, Chongqing 408300, China
Abstract：OBJECTIVE To observe the effect of miR-204-5p on oxygen-glucose deprivation and reoxygenation (OGD/R) injury in SH-SY5Y cells and to explore its possible mechanism from inflammatory/apoptotic pathways. METHODS SH-SY5Y cells were divided into control group, OGD/R group, OGD/R+miR-204-5p mimic group, OGD/R+miR-204-5p inhibitor group, OGD/R+miR-204-5p mimic negative control group, OGD/R+miR-204-5p inhibitor negative control group. Cell viability was measured by MTT assay, cell apoptosis was detected by flow cytometry and TUNEL staining. The changes of IL-10, IL-1β, TNF-α, PGE2 were accessed by enzyme-linked immunosorbent assay. RT-PCR was used to detect the expression of miR-204-5p and Western blot was used to measure the expressions of inflammatory and apoptosis-related proteins (COX-2, Bcl-2, Bax). RESULTS Compared with control group, the expression of miR-204-5p was decreased obviously, COX-2, Bax, IL-1β, TNF-α, PGE2 were up-regulated significantly and Bcl-2, IL-10 were down-regulated, and the cell injury was significantly aggravated in OGD/R group. Compared with the OGD/R group, miR-204-5p mimic had enhanced cell viability and IL-10 content significantly, declined apoptosis rate and IL-1β, TNF-α, and PGE2 content. Simultaneously, reduced COX-2 and Bax protein expression, and increased Bcl-2 protein expression were detected in miR-204-5p mimic group. CONCLUSION miR-204-5p has a protective effect on OGD/R-induced SH-SY5Y cell injury, its mechanism may be associated with the reduction of cellular inflammation and apoptosis.
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2022, Vol. 57 
