Simultaneous Quantification of 3 Non-volatile Components and Fingerprints Comparison of Asarum heterotropoides Commercial Herbs Using RP-HPLC
WEN Xiao-yin1, WU Hao1, YAN Peng1, SHEN Shui-rong2, ZHONG Shan1, LIU Xiao-dong1,3*, LAI Ke-fang1*
1. State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510182, China; 2. Guangdong Institute of Nanshan Pharmaceutical Innovation, Guangzhou 510530, China; 3. Duke Global Health Institute, Duke Medical Center, Duke University, Durham, North Carolina, 27708, the United States
Abstract：OBJECTIVE To establish the simultaneous quantification method of 3 non-volatile components and fingerprints comparison of Asarum heterotropoides and provide a scientific method for the quality standards and quality control of A. heterotropoides. METHODS Twenty-one batches of A. heterotropoides were extracted by heating and refluxing with 70% ethanol and analyzed by HPLC on Agilent SB-C18 column (4.6 mm×250 mm, 5 μm), with the mobile phase consisted of acetonitrile and aqueous with a gradient elution for simultaneous quantification of 3 non-volatile components, fingerprints comparison and Cluster analysis. The flow rate was 1.0 mL·min-1, the column temperature maintained at 30 ℃, and the detection wavelengths were set at 285 nm. RESULTS Asarinin, sesamin, and kakuol showed good linearity (r=0.999 9) in the ranges of 0.015 625-0.5 mg·mL-1, 0.015 625-0.5 mg·mL-1 and 0.006 25-0.2 mg·mL-1, respectively. The average recoveries were 98.31%, 97.12% and 95.54%, with RSD 1.59%, 1.73% and 2.46%, respectively. The contents of the 3 non-volatile components in the 21 batches of samples were in a range of 0.630 6-3.123 3 mg·g-1 for asarinin, 0.255 4-1.236 8 mg·g-1 for sesamin, and 0.071 4-0.469 4 mg·g-1 for kakuol. The similarity coefficient of the fingerprints of 21 batches samples were above 0.989. CONCLUSION There are certain differences in the content of 3 non-volatile components in different batches of A. heterotropoides samples, but the content of asarinin, as the index component of A. heterotropoides, meets the requirements of the Chinese Pharmacopoeia. The established simultaneous quantification method and fingerprints are highly sensitive with strong specificity and it can be used efficiently in the quality control of A. heterotropoides.
YU J J. The analgesic mechanism study of Asarum by regulating TRPV1 ion channel[D]. Nanjing: Nanjing University of Chin Med(南京中医药大学), 2019.
[5]
LI Y M, ZHOU Z X, HAN L T, et al. Mechanism of asarum-dried ginger drug pair based on network pharmacology[J]. Lishizhen Med Mater Med Res(时珍国医国药), 2019, 30(3):726-730.
[6]
LIU J, MENG L, YUAN Z M, et al. Preliminary study on material basis of Asari Radix et Rhizoma against asthma and cough[J]. Her Med(医药导报), 2012, 31(11):1412-1415.
[7]
WU J B, CHU X P, ZHANG Y Y, et al. Screening of the anti-asthma active sites for the drug partnership containing Fructus schisandrae and Asarum[J]. Chin Arch Tradit Chin Med(中华中医药学刊), 2013, 31(1):121-123.
[8]
SUN Q H, ZHANG J, LI Z Z, et al. Action mechanism of Mahuang Xixin Fuzi decoction for mice with influenza based on metabolomics information[J]. China J Chin Mater Med(中国中药杂志), 2017, 42(4):763-771.
[9]
ZENG L D, PENG H L, SONG G H, et al. Inhibitory activities of ethanol extracts from 5 species of plants and their compounds against Peronophythora litchi Chen[J]. J South Agric(南方农业学报), 2016, 47(8):1332-1337.
[10]
SUN L, HU W Z, LIU C H, et al. Advances in functional ingredients in Liao Asarum[J]. Sci Tech Food Ind(食品工业科技), 2016, 37(4):391-394.
[11]
REN T, SONG T Y, MU Y Y, et al. Optimization of extraction process and protective effect of cellular senescence from Asarum polysaccharides[J]. Sci Tech Food Ind(食品工业科技), 2019, 40(11):33-39,45.
[12]
QIN Z N, WANG M X, SHI N N, et al. Rationality of Asari Radix et Rhizoma in Qingfei Paidu Decoction based on literature analysis[J]. China J Chin Mater Med(中国中药杂志), 2020, 45(7):1515-1520.
[13]
WU H, WEN X Y, YAN P, et al. Research progress on chemical constituents and pharmacological effects of Asari Radix et Rhizoma[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2021,27(4):186-195.
[14]
AFROZ M, ZIHAD S M N K, UDDIN S J, et al. A systematic review on antioxidant and antiinflammatory activity of Sesame (Sesamum indicum L.) oil and further confirmation of antiinflammatory activity by chemical profiling and molecular docking[J]. Phytother Res, 2019, 33(10):2585-2608.
[15]
MAJDALAWIEH A F, MASSRI M, NASRALLAH G K. A comprehensive review on the anti-cancer properties and mechanisms of action of sesamin, a lignan in sesame seeds (Sesamum indicum)[J]. Eur J Pharmacol, 2017, 815:512-521.
[16]
ZHANG L L, GUAN Z Z, ZHANG J K. Experimental study of the effects of asarinin on immunosuppression activity in vitro[J]. Chin J Cardiol(中华心血管病杂志), 2003, 31(6):47-50.
[17]
CAO S, LI Y M, DAI W Q, et al. Content determination of asarinin in different parts of Asarum by HPLC[J]. J Hubei Univ Tradit Chin Med(湖北中医药大学学报), 2018, 20(4):42-44.
[18]
YU J, ZHANG Y F, SHANG M Y, et al. Chemical constituents from the roots and rhizomes of Asarum heterotropoides var. mandshuricum and the in vitro anti-inflammatory activity [J]. Molecules, 2017, 22(1):125.
[19]
QI W, WANG L L, WEN H, et al. Isolation and identification of chemical constituents in roots and rhizomes of Asarum heterotropoides Fr.Schmidt var. mandshuricum (Maxim.) Kitag.[J]. J Shenyang Pharm Univ(沈阳药科大学学报), 2014, 31(9): 681-686, 691.
[20]
HUANG J, WANG H Q, ZHANG C, et al. A new tetrahydrofuran-type lignan with anti-inflammatory activity from Asarum heterotropoides Fr. Schmidt var. mandshuricum[J]. J Asian Nat Prod Res, 2014, 16(4):387-392.
[21]
QUANG T H, NGAN N T, MINH C V, et al. Anti-inflammatory and PPAR transactivational effects of secondary metabolites from the roots of Asarum sieboldii [J]. Bioorg & Med Chem Lett, 2012, 22(7):2527-2533.
[22]
CAO C, WANG J Z, WANG L, et al. Simultaneous determination of seven principal constituents in Asari Radix et Rhizoma by HPLC[J]. J Chin Pharm Sci(中国药学英文版), 2015, 24(8): 530-537.
[23]
WEI Q C, LV H Z. Isolation and identification of chemical constituents from Asarum sieboidii var seoulense Nakai[J]. Lishizhen Med Mater Med Res(时珍国医国药), 2010, 21(3): 676-677.
[24]
ZHU M. Isolation and property study on the polysaceharide of Herba Asari[D]. Changchun: Northeast Normal Univ(东北师范大学), 2007.
2021, Vol. 56 
