Effects of Eupolyphaga sinensis Walker Hemolymph on Proliferation and Apoptosis of Human Erythroleukemia HEL Cells
ZHANG Yue1,2, MA You-fen2, YU Jia2, DAI Ren-huai1*, LUO Heng2*
1. Institute of Entomology, The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Guizhou University, Guizhou 550025, China; 2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Plants, Guiyang 560014, China
Abstract：OBJECTIVE To explore the effect of Eupolyphaga sinensis Walker hemolymph (ESWH) on the proliferation and apoptosis of human erythroleukemia HEL cells. METHODS The cells were treated with different concentrations (10-60 mg·L-1) of ESWH in vitro. Cell viability was assessed by MTT assay, the morphological changes of apoptotic HEL cells were observed by Hoechst 33258 and Annexin V-FITC fluorescence staining, and the apoptosis rate was evaluated by Annexin/PI method. Furthermore, apoptosis and cell cycle distribution were analyzed with flow cytometry, and RT-qPCR and Western blot assay were employed to detect mRNA and protein expression levels of Bcl-2, Bax, caspase-3 and caspase-8, Cyt C. RESULTS ESWH inhibited HEL cells proliferation in a time and dose-dependent manner. ESWH could induce G2/M phase cell cycle arrest in HEL cells, promote cell apoptosis, down-regulate the mRNA and protein expression levels of Bcl-2 (P<0.01), and up-regulate the protein expression levels of Bax, Cyt C, cleaved caspase-3 and cleaved caspase-8 (P<0.01). CONCLUSION ESWH could inhibit proliferation and induce apoptosis of human erythroleukemia HEL cells, and the mechanism may be associated with activating the mitochondria-dependent pathway.
张越, 马佑芬, 余佳, 戴仁怀, 骆衡. 中华真地鳖血淋巴对人红白血病HEL细胞增殖及凋亡的影响[J]. 中国药学杂志, 2021, 56(7): 539-545.
ZHANG Yue, MA You-fen, YU Jia, DAI Ren-huai, LUO Heng. Effects of Eupolyphaga sinensis Walker Hemolymph on Proliferation and Apoptosis of Human Erythroleukemia HEL Cells. Chinese Pharmaceutical Journal, 2021, 56(7): 539-545.
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2021, Vol. 56 
