Effect and Possible Mechanism of Kaempferol on Acute Kidney Injury in LPS-Stimulated Mice
CHENG Xiao, YANG Ying-lin, LI Wei-han, LIU Man, WANG Yue-hua*, DU Guan-hua*
Beijing Key Laboratory of Drug Target Identification and New Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
Abstract：OBJECTIVE To investigate the effect of kaempferol (KAE) on acute kidney injury induced by lipopolysaccharide (LPS) in mice and its mechanism. METHODS BALB/c mice were randomly divided into normal control group, LPS model group, KAE 25, 50, 100 mg·kg-1 treatment groups. After 7 d of administration, mice in LPS model group and KAE administration groups were injected with LPS 5 mg·kg-1 intraperitoneally, and serum and kidney tissues were collected for detection after LPS injection 6 h. The levels of urea nitrogen (BUN) and creatinine (CRE) in serum were measured to evaluate renal function; the levels of IL-1β, IL-6, TNF-α and ICAM-1 in kidney tissues were detected by ELISA, and the expression of inflammatory pathway-related proteins was detected by Western blot. RESULTS Compared with the normal control group, the levels of BUN and CRE in serum of LPS model group were increased significantly, while KAE treatment decreased the levels of BUN and CRE in a dose-dependent manner. Compared with the normal control group, the levels of inflammatory related factors and proteins IL-1β, IL-6, TNF-α, ICAM-1, HMGB1, TLR4 and P2X7 in kidney tissues were significantly increased, while KAE treatment could decrease the release of proinflammatory factors and adhesion molecules in kidney tissue in a dose-dependent manner, and down-regulate the expression of inflammatory proteins including HMGB1, TLR4 and P2X7. CONCLUSION Kaempferol can improve renal function and inhibit acute inflammatory responses induced by LPS stimulation in mice, and the mechanism may be related to down-regulation of HMGB1, TLR4 and P2X7 inflammatory proteins.
程笑, 杨滢霖, 李伟瀚, 刘漫, 王月华, 杜冠华. 山柰酚对LPS诱导小鼠急性肾损伤作用及机制探讨[J]. 中国药学杂志, 2020, 55(17): 1439-1443.
CHENG Xiao, YANG Ying-lin, LI Wei-han, LIU Man, WANG Yue-hua, DU Guan-hua. Effect and Possible Mechanism of Kaempferol on Acute Kidney Injury in LPS-Stimulated Mice. Chinese Pharmaceutical Journal, 2020, 55(17): 1439-1443.
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2020, Vol. 55 
