Abstract：OBJECTIVE To investigate and apply the bioaccessibility of Cd in traditional Chinese medicines (TCMs) to human health risk assessment, and provide reference for the formulation of limit standards. METHODS The contents of Cd in Coptis chinensis Franch., Ligusticum chuanxiong Hort., Lindera aggregate (Sims) Kosterm., and Curcuma kwangsiensis S.G. Lee et C.F. Liang were determined by inductively coupled plasma mass spectrometry (ICP-MS). The bioaccessibility of Cd in TCMs was investigated by in vitro physiologically based extraction test(PBET) digestion method. And the target hazard quotient (THQ) was applied to assess the health risks of Cd contents. RESULTS The bioaccessibility of Cd for Coptis chinensis Franch., Ligusticum chuanxiong Hort., Lindera aggregate (Sims) Kosterm., and Curcuma kwangsiensis S.G. Lee et C.F. Liang was 49.0%, 40.0%, 44.9% and 65.7%, respectively. The results of risk assessment showed that before extraction, THQ values of Cd for Coptis chinensis Franch., Ligusticum chuanxiong Hort., Lindera aggregate (Sims) Kosterm., and Curcuma kwangsiensis S.G. Lee et C.F. Liang were 1.895, 1.045, 1.446 and 1.653,respectively; after extraction, THQ values of Cd for the four types of TCMs were 0.929, 0.418, 0.649 and 1.077, respectively. CONCLUSION Cd in the four types of TCMs cannot be completely absorbed in the artificial gastrointestinal phase, and the risks of Cd are reduced after extraction. Except THQ for Curcuma kwangsiensis S.G. Lee et C.F. Liang, the THQ values are below the value of 1, indicating very low health risks for the other three types of TCMs after extraction. In this study, the bioaccessibility of heavy metals in TCMs is applied to the health risk assessment model in order to provide a scientific basis for objectively evaluating the health risks of heavy metals in TCMs and formulating a scientific and reasonable heavy metal limit standards.
左甜甜, 屈浩然, 陈虹, 康帅, 孙磊, 金红宇, 马双成. 中药材中镉的生物可给性在风险评估中的应用[J]. 中国药学杂志, 2020, 55(15): 1243-1247.
ZUO Tian-tian, QU Hao-ran, CHEN Hong, KANG Shuai, SUN Lei, JIN Hong-yu, MA Shuang-cheng. Application of Bioaccessibility in Health Risk Assessment of Cd in Traditional Chinese Medicines. Chinese Pharmaceutical Journal, 2020, 55(15): 1243-1247.
CHEN X, WANG K, WANG Z, et al. Effects of lead and cadmium co-exposure on bone mineral density in a Chinese population . Bone, 2014, 63(6):76-80.
[2]
TCHOUNWOU P B, YEDJOU C G, PATLOLLA A K, et al. Heavy metals toxicity and the environment. Exp, 2012, 101(suppl):133-164.
[3]
XIE D Z, ZHAN X J, SHU X R, et al. In vitro antibacterial activity of traditional Chinese medicine such as coptidis rhizome on drug-resistant neisseria gonorrhoeae . Chin J Nosocomiol (中华医院感染学杂志), 2016, 26(10):2193-2195.
[4]
RUBY M V, SCHOOF R, BRATTIN W, et al. Advances in evaluating the oral bioaccessibility of inorganics in soil for use in human health risk assessment . Environ Sci Technol, 1999,33 (21):3697-3705.
[5]
ZHOU L Y, YUE H, LI X, et al. Bioaccessibility of heavy metal in wild Artemisia annua and its health risk assessment . Chin J Chin Mater Med (中国中药杂志), 2015, 40 (10):1904-1907.
[6]
LI F Q, XU J, LIU S N, et al. Application of bioaccessibility in the risk assessment of food contaminants. Chin J Food Hygi (中国食品卫生杂志), 2011,23(1):17-22.
[7]
RUBY M V, DAVIS A, LINK T E, et al. Development of an in vitro screening test to evaluate the in vivo bioaccessibility of ingested mine-waste lead. Environ Sci Technol, 1993, 27 (13):2870-2877.
[8]
LIAO W, WANG G, ZHAO W B, et al. Bioaccessibility of mercury in fish based on an in vitro physiologically-based extraction test . Environ Prot Sci(环境保护科学), 2018, 44 (2):103-108.
[9]
USEPA. Risk-Based Concentration Table . Washington DC:United States Environmental Protection Agency, 2000. KHAN S, CAO Q, ZHENG Y M, et al. Health risks of heavy metals in contaminated soil and food crops irrigated with wastewater in Beijing, China. Environ Pollut, 2008, 152(3):686-692. MAHMOOD A, MALIK R N. Human health risk assessment of heavy metals via consumption of contaminated vegetables collected from different irrigation sources in Lahore, Pakistan . Arab J Chem, 2013, 7(1):91-99. LI Y, ZHANG M K. A comparison of physiologically based extraction test (PBET) and single-extraction methods for release of Cu, Zn, and Pb from mildly acidic and alkali soils . Environ Sci Pollut Res Int, 2013, 20 (5):3140-3148. SIALELLI J, URQUHART G J, DAVIDSON C M, et al. Use of a physiologically based extraction test to estimate the human bioaccessibility of potentially toxic elements in urban soils from the city of Glasgow, UK . Environ Geochem Health, 2010, 32(6):517-527. VERHEYEN L, DEGRYSE F, NIEWOLD T, et al. Labile complexes facilitate cadmium uptake by caco-2 cells . Sci Total Environ, 2012, 426:90-99. ZHANG Z Z, CHENG B, LI R, et al. Status and health risk assessment of vegetable heavy metal contamination in Taiyuan City . Chin Agric Bull(中国农学通报), 2016, 32(10):169-175. REN Y J, MA J J. Pollution status and health risk analysis of heavy metal in vegetables in Qinhuangdao City . J Safe Environ(安全与环境学报),2013,13(2):79-84. ZUO T T, LI Y L, CHEN P, et al. Determination and preliminary risk assessment of heavy metals and harmful elements in American ginseng, the fruit of Chinese wolfberry and hawthorn . Chin J Pharm Anal (药物分析杂志), 2016,36(11):2016-2021. NGO D M, HOUGH R L, LE T T, et al. Assessing dietary exposure to cadmium in a metal recycling community in Vietnam:age and gender aspects . Sci Total Environ, 2012, 416(2):164-171.
2020, Vol. 55 
