1.Xinjiang Medical University, a. the Central Lab; b. Pharmacy College, Urumq 830011, China; 2. Affiliated Tumor Hospital of Xinjiang Medical University, Urumq 830011, China; 3. Xinjiang Ailexin Pharmacy Company, Urumq 830011, China
Abstract��OBJECTIVE To study the absorption characteristics of allicin in rat intestinal tract.METHODS An HPLC method was established to determine the content of allicin in rat entericus, and Ussing chamber system was used to investigate the absorption characteristics of garlic spicy element of different concentrations in the duodenum, jejunum, ileum and colon of rats. RESULTS An HPLC method was established to determine the content of allicin in rat intestinal liquid.The absorption rate constants of allicin in various intestinal segments of rats in the experimental concentration range increased with the increase of allicin concentration.Allicin showed linear absorption in different intestinal segments of rats, with regression correlation coefficients of greater than 0.96, indicating zero order absorption. CONCLUSION Allicin has different absorption characteristics in different intestinal segments of rats and can be absorbed as prototype.
��ѩ��, ����, Ф�Ŀ�, ����ϼ, �¼�. ��˹��ϵͳ�о����������ڴ���ͬ���ε���������[J]. �й�ҩѧ��־, 2019, 54(8): 620-623.
MAXue-hong, WANGJian, XIAOWen-jun, LIXin-xia, CHENJian. Absorption Study of Allicin in Different Intestinal Segments in Rats by Ussing Chamber System. Chinese Pharmaceutical Journal, 2019, 54(8): 620-623.
ICHIKAWA M, IDE N, YOSHIDA J, et al. Determination of organosulfur compounds in garlic by high-performance liquid chromatography[J]. J Agric Food Chem, 2006, 54(5):1535-1540.
[2]
Editorial Board of Chinese Materia Medica. Chinese Materia Medica(��ҩ��)[M]. Shanghai:Shanghai Science Technology Press,1999,22:36-43.
[3]
MIRON T, SIVARAMAN H, RABINKOV A, et al. A method for continuous production of allicin using immobilized alliinase[J]. J Anal Biochem, 2006, 351(1): 152-154.
[4]
WANG L, SONG M, HANG T J, et al. HPLC Tandem-mass spectrometric analysis of the chemical components and decomposition products of allicin extract of garlic [J]. Acta Pharm Sin(ҩѧѧ��), 2009, 44(1): 74-79.
[5]
GUO N, WU X P, YU L, et al. In vitro and in vivo interactions between fluconazole and allicin against clinical isolates of fluconazole-resistant Candida albicans determined by alternative methods [J]. FEMS Immunol Med Microbiol, 2010, 58(2): 193-201.
[6]
BERG�HS R, SIESS M H, ARNAULT I, et al. Comparison of the chemopreventive efficacies of garlic powders with different alliin contents against aflatoxin B1 carcinogenicity in rats[J]. Carcinogenesis, 2004, 25(10):1953-1959.
[7]
MOUSA A S, MOUSA S A. Anti-angiogenesis efficacy of the garlic ingredient alliina nd antioxidants: role of nitricoxideand p53[J]. Nutr Cancer, 2005, 53(1): 104-110.
[8]
CHEN F, ZHU Z H, LIU S H, et al. In vitro antibacterial effects of alliin, alliinase and alliin+alliinase against methicillin-resistant Staphylococcus aureus[J]. J Xi��an Jiaotong Univ(Med Sci)(������ͨ��ѧѧ��:ҽѧ��), 2011, 32(2): 190-192.
[9]
FENG Y, ZHU X, WANG Q, et al. Allicin enhances host pro-inflammatory immune responses and protects against acute murine malaria infection[J]. Malar J, 2012, 8(11): 268-270.
[10]
LENNERNÄS H. Animal data: the contributions of the Ussing Chamber and perfusion systems to predicting human oral drug delivery in vivo[J]. Adv Drug Deliv Rev, 2007, 59(11): 1103-1120.
2019, Vol. 54 
