Metabolomics in the Nitroglycerin-Induced Migraine Rats Intervened by Flunarizine
WANG Qing-qing1a, LIU Yu-min2, LIN Xiao1a,1b, FENG Yi1b, SHEN Lan1a,1b*
1a. School of Traditional Chinese Medicine, 1b. Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine,Ministry of Education,Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2. Instrumental Analysis Centre, Shanghai Jiaotong University, Shanghai 201100, China
Abstract��OBJECTIVE To study the mechanism of flunarizine in the nitroglycerin-induced migraine model rats by metabonomics. METHODS The nitroglycerin-induced migraine rat model was used and the brain samples and serum samples were obtained at 30, 60, and 90 min after model establishment or drug administration. After the isolation of TCC sites was performed,GC-TOF-MS was used to analyze the metabolic status of rat serum and TCC sites in different groups. RESULTS The metabolic status of the model group and the flunarizine group differed from that of the control group at the same time point.The metabolic state that compared with the model group or the flunarizine group at different time points changes continuously with time. Compared with those in the model group, L-asparagine, 5-methoxytryptamine, ��-lactose, ribitol, arachidonic acid, and glycerol in the serum samples and the phosphoglycolic acid, erythrose, inosine, glyceric acid, and D-glucose in the TCC samples were changed in the flunarizine group, indicating that the energy metabolism pathways and amino acid metabolic pathways were involved in the mechanism of flunarizine intervening migraine. CONCLUSION Flunarizine may improve metabolic disorders in nitroglycerin-induced migraine model rats by affecting energy metabolism and amino acid metabolism.
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WANG Qing-qing, LIU Yu-min, LIN Xiao, FENG Yi, SHEN Lan. Metabolomics in the Nitroglycerin-Induced Migraine Rats Intervened by Flunarizine. Chinese Pharmaceutical Journal, 2019, 54(3): 193-199.
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2019, Vol. 54 
