Abstract��OBJECTIVE To isolate and identify the total alkaloids from Aconitum excelsum and to study the therapeutic mechanism of the total alkaloids from Aconitum excelsum on chronic bronchitis in rats. METHODS The total alkaloids from Aconitum excelsum was isolated by TLC and the structures of compounds were elucidated by a variety of spectral techniques. The rat model of chronic bronchitis (CB) was established with combined utilization of bacillus calmette-guerin and lipopolysaccharide, dexamethasone tablets and total alkaloids from Aconitum excelsum (high and low dose) were ig administrated. The pathological changes of lung tissue in rats were observed by HE and the level changes of interleukin-1 (IL-1), interleukin-8 (IL-8) and tumor necrosis factor-�� (TNF-��) in serum were determined by ELISA method. RESULTS Seven alkaloids were identified from the total alkaloids of Aconitum excelsum. The administration of total alkaloids from Aconitum excelsum could decrease the degree of infiltration, epithelial cell shedding, bronchial secretion, inhibit the proliferation of bronchial epithelial cells of the rat bronchial inflammatory cells, and make the bronchial diameter increases and ventilation. The total alkaloids could significantly inhibit the increase of IL-1, IL-8 and TNF-�� in the serum of rats with CB, compared with the model group with the statistical significance (P<0.05). CONCLUSION The total alkaloids from Aconitum excelsum have the better treatment effect on CB, which may be related to its main chemical components, namely lappaconitine (1), ranaconitine (2), septentriod-ine (3), 6-demethyldelsodine (4), 6-methylumbrofine (5), 8-methyl-10-hydroxylycoctonine (6) and 8-methyllycoctonine (7).
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HONG Yan, WANG Qing-hu, BIi-LI-GE-TU, BAO Wen-qiang, YU Hua. Isolation and Identification of Total Alkaloids from Mongolian Medicine Aconitum excelsum and Its Therapeutic Mechanism on Chronic Bronchitis in Rats. Chinese Pharmaceutical Journal, 2019, 54(2): 91-97.
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2019, Vol. 54 
