Therapeutic Effect of Arginyl-Fructosyl-Glucose on Diabetic Nephropathy Mice and Correlation of Gene TGF-��1 and Collagen-IV
SHE Xin-xin1, GAO Ming-tong1, LIU Xing-long1, ZENG Lu-lu1, DING Chuan-bo1, ZHAO Ting1, ZHENG Yi-nan1, LIU Wen-cong1*, XU Xiao-hua2*
1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; 2. Department of Nephrology, China-Japan Friendship Hospital, Jilin University, Changchun 130033,China
Abstract��OBJECTIVE To investigate the mechanism and the protective effect of arginyl-fructosyl-glucose(AFG) on the kidney of diabetic nephropathy(DN) mice and the correlation between TGF-��1 and collagen ��. METHODS Twelve mice were randomly selected as control group; all others were induced by streptozotocin (STZ). The mice were randomly divided into 5 groups, each of which has 12 mice, DN model group, metformin group, 10,20,40 mg��kg-1 AFG group; each group was treated for 5 weeks, during when mice were weighed weekly and blood glucose concentration were measured. At the end of 5 weeks, the final body weight and the renal index of the mice was calculated; 24 h urinary protein and blood biochemical indexes were recorded (TC, TG, HDL-C, SCr, BUN, SOD, GSH, CAT, T-AOC); the pathological changes of the kidney was observed using HE staining. The expressions of TGF-��1 and collagen �� mRNA in kidneys were detected by real-time fluorescence quantitative PCR (RT-PCR). RESULTS The levels of urinary protein, TC, TG and HDL-C in the AFG group were significantly lower than those in the control group. The contents of SOD, GSH, CAT and T-AOC in the AFG-treatment group were significantly higher than those in the control group. Pathological condition was relieved; the mRNA expression of TGF-��1 and collagen �� in the kidneys of each dose group was significantly lower than that of the control group. CONCLUSION AFG protects the kidneys by reducing the total amount of 24 h urinary protein, decreasing renal pathological damage and decreasing the expression of TGF-��1 and collagen �� genes in kidney.
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2018, Vol. 53 
