Abstract��OBJECTIVE To investigate the protective effect and possible mechanism of Rhizoma Coptis(RC) on lipopolysaccharide(LPS)-induced inflammatory injury in rat hepatocytes(BRL). METHODS LPS-induced BRL cells injury model was established in vitro, then the damaged cells were given different interventions and treatment with 0.175, 0.1 mg�� mL-1 RC aqueous extract as the test drug, and dexamethasone(Dex) as positive control drug. The optimal test doses of LPS and RC aqueous extract were selected and determined by cell counting kit-8(CCK-8), the cellular apoptosis rate was determined by flow cytometry, TLR4/NF-��B and TLR4/IRF3 signaling pathways and the mRNA level of related inflammatory mediators(TNF-��, IL-1��, IL-6) were detected by RT-PCR, the NF-��B p65 protein expression was analysed by Western blot and immunofluorescence techniques. RESULTS ��Compared with normal control group, 0.1 mg��mL-1 LPS affected on BRL cells for 24 h, the cell survival rate was decreased significantly(P<0.01), the apoptotic rate increased significantly(P<0.01), the mRNA level of TLR4, NF-��B, IRF3, TNF-��, IL-1��, IL-6 were significantly increased(P<0.01), and the NF-��B p65 protein expression was increased. ��Compared with the model group, 0.1 and 0.175 mg��mL-1 RC affected on LPS-induced BRL cells for 24 h, the survival rate of BRL cells was increased significantly(P<0.05), the apoptotic rate decreased significantly(P<0.01), the mRNA level of TLR4, NF-��B, IRF3, TNF-��, IL-1��, IL-6 and the NF-��B p65 protein expression were decreased significantly(P<0.01). CONCLUSION Rhizoma Coptis has obviously protective effect on LPS-induced inflammatory injury in rat hepatocytes(BRL), the mechanism of which may be related with inhibiting apoptosis, reducing the release of inflammatory factors such as TNF-����IL-1�� and IL-6, blocking NF-��B p65 protein nuclear translocation, interfering the R4/NF-��B and TLR4/IRF3 signaling pathway.
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HAN Chun-yang, SUN Tao-tao, XU Jing, LIU Ya-yun, LIU Cui-yan. Protective Effect of Rhizoma Coptis on LPS-induced Injury of Rat Liver Cells. Chinese Pharmaceutical Journal, 2018, 53(11): 869-875.
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