Analysis of Difference of Chemical Constituents in Magnolia officinalis var. biloba Rehd. et Wils from Different Commercial Specifications by LC-Triple TOF MS/MS
ZHAO Hui, YAN Ying, ZOU Li-si, LIU Xun-hong*, CHAI Chuan, HUA Yu-jiao, WANG Sheng-nan
Nanjing University of Chinese Medicine, Nanjing 210023, China
Abstract��OBJECTIVE To analyze the differences in chemical composition of Magnolia officinalis var. biloba Rehd. et Wils from different commercial specifications. METHODS The difference in chemical constituents of Magnolia officinalis var. biloba Rehd. et Wils from different commercial specifications was analyzed by LC-Triple TOF MS/MS combined with multivariate statistical analysis. Through analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, and noise filtering. Principal component analysis(PCA)and partial least-squares discriminant analysis(PLS-DA)were used for data processing. The components were identified according to MS accurate mass and MS/MS spectrometry fragmentation information, combined with the database search and literature. RESULTS The chemical constituents in Magnolia officinalis var. biloba Rehd. et Wils from different commercial specifications were clearly distinguished. Twenty-two chemical constituents had significant differences. Among them, 10 kinds of common different chemical constituents, such as leonoside A, syringaresinol-4'-O-��-D-glucopyranoside, magnolignan D, magnolignan A or C, randaiol, honokiol, obovatol, magnolignan G,(��)9-HODE and piperitylmagnolol, presented different changing laws. CONCLUSION This study can provide references for establishment of a mathematical model for prediction of the specifications of chemical information of Magnoliae Officinalis Cortex based on metabolomics and comprehensive evaluation of the quality of medicinal herbs.
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ZHAO Hui, YAN Ying, ZOU Li-si, LIU Xun-hong, CHAI Chuan, HUA Yu-jiao, WANG Sheng-nan. Analysis of Difference of Chemical Constituents in Magnolia officinalis var. biloba Rehd. et Wils from Different Commercial Specifications by LC-Triple TOF MS/MS. Chinese Pharmaceutical Journal, 2017, 52(19): 1720-1726.
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YU S X, YAN R Y, LIANG R X, et al. Bioactive polar compounds from stem bark of Magnolia Officinalis [J]. Fitoterapi, 2011, 83(2): 356-361.
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ZHANG H M, REN H, HE Y, et al. Effects of Magnolia officinalis with different extraction techniques on analgesia function in mice [J]. Nat Sci J Chengdu Univ(�ɶ���ѧѧ��:��Ȼ��ѧ��), 2013,32(3): 235-237.
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SHI L, ZHANG C C, GUO L P, et al. Research on standard of Magnoliae officinalis Cortex commercial specification and grade [J]. China J Chin Mater Med(�й���ҩ��־), 2015, 40(3): 450-454.
[9]
YU S X, YAN R Y, ZHANG C X, et al. The investigation of Magnoliae Officinalis Cortex commercial specification [J]. J Chni Med Mater(��ҩ��), 2011, 34(4): 522-524.
[10]
FENG J, WU X N, YU C H, et al. Determination of honokiol and magnolol in different specifications of Magnolia officinalis by HPLC [J]. Chin Arch Tradit Chin Med(�л���ҽҩѧ��), 2011,29(6):1338-1339.
[11]
LI J S, WU B, CAO Z Q, et al. Chinese herbal medicine productspecifications change [J]. J Tradit Chin Med(��ͳ��ҽҩ), 2012, 2(3):41-42.
[12]
QIN X M, KONG Z K, ZHANG L Z, et al. Interpretation of ��assessing quality by distinguishing TCMM features��and research ideas about commodity specifications [J].Chin Tradit Herb Drugs(�в�ҩ), 2012, 43(11): 2093-2098.
[13]
LI C Y, WANG Z, WANG P, et al. Therapeutic material basis and action mechanism of Chinese materia medica based on metabonomic technology [J]. Chin Tradit Herb Drugs(�в�ҩ), 2013, 44(7): 918-922.
[14]
MI X, LI Z Y, QIN X M, et al. Monitoring of chemical components with different color traits of Tussilago farfara using NMR-based metabolomics [J]. Acta Pharm Sin(ҩѧѧ��), 2013, 48(11): 1692-1697.
[15]
HOU Y, MA Y, ZOU L S, et al. Analysis of difference in chemical constituents of Radix Pseudostellariae from different germplasms by UPLC-Triple TOF-MS/MS [J]. Chin Pharm J(�й�ҩѧ��־), 2015, 50(13): 1104-1110.
[16]
LUO Y Y, LIU J X, LIU X H, et al. Analysis of chemical constituents in Polygoni Multifori Radix rocessed by different methods using UPLC-QTOF-MS [J]. J Instr Anal(��������ѧ��), 2017, 36(1): 73-79.
[17]
YU X W, WU Q, LV W,et al. Metabonomics study of lung cancer cells based on liquid chromatography-mass spectrometry [J]. Chin J Chromatogr(ɫ��), 2013, 31(7): 691-696.
[18]
LI A P, LI Z Y, X J, et al. Chemical characterization of different vinegars by NMR-based metabolomics approach [J]. Food Sci(ʳƷ��ѧ), 2013,34(12): 247-253.
[19]
TRYGG J, HOLMES E, LUNDSTEDT T. Chemometrics in metabonomics [J]. J Proteome Res, 2007,6(2): 469-479.
[20]
TIAN C X,XU X P,LIAO L Y, et al. Separation and identification of chlorogenic acid and related impurities by high performance liquid chromatography-tandem mass spectrometry [J]. Chin J Chromatogr(ɫ��), 2007, 25(4): 496-500.
[21]
HASEGAWA T, FUKUYAMA Y, YAMADA T, et al. Structures of magnolosides B and C, novel phenylpropanoid glycosides with allopyranose as core the sugar unit [J]. Chem Pharm Bull, 1988, 36(3): 1245-1248.
[22]
XUE Z Z, YAN R Y, YU S X, et al. Determination of six active compounds in commercial samples of Magnoliae Officinalis Cortex by HPLC-DAD [J]. Chin J Exp Tradit Med Form(�й�ʵ�鷽��ѧ��־), 2014, 20(22): 45-49.
[23]
SONG Y, FENG X F. UPLC-MS/MS Analysis of the constituents in the roots of A. sessiliflorus and comparison with A. gracilistylus and A.senticosus [J]. Chin J Pharm Anal(ҩ�������־), 2014, 34(6): 958-965.
[24]
HASEGAWA T, FUKUYAMA Y, YAMADA T, et al. Isolation and structure of magnoloside A, a new phenylpropanoid glycoside from Magnolia obovata Thunb [J]. Chem Lett, 1988(1): 163-166.
[25]
WANG L, YUANA K, YU W W, et al. Evaluation and discrimination of Magnoliae Officinalis Cortex produced in Zhejiang Province(Wen-Hou-Po)by UPLC-DAD-TOF-MS fingerprint [J]. Nat Prod Commun, 2010, 5(10): 1631-1638.
[26]
CHEN S, QIU H H, ZHOU T, et al. Constituents determination in n-butanol part from Magnoliae Officinalis Cortex before and after ginger mix-frying by HPLC-MS [J]. China Pharm(�й�ҩʦ), 2015, 18(5): 761-765.
[27]
HAN L, SHI Z F, LIN H Q. Determination of the constituents of Magnoliae Officinalis Cortex by UPLC/Q-TOF-MS/MS [J]. Chin Tradit Patent Med(�г�ҩ), 2013, 35(4): 766-769.
[28]
WANG J J. Identification of ESI-MS decomposition of magnolol and honokiol and its extracellular metabolites [D]. Huibei: University of Hubei, 2008.
[29]
LIU Y. Study on the mass spectrometric analytical method and correlation spectroscopy of LC-MS/NMR of phenylethanoid glycoside and their complex mixtures[D]. Beijing: Institute of Materia Medica, Chinese Academy of Medical Sciences ��Peking Union Medical College, 2009.
2017, Vol. 52 
