Accumulation of Main Chemical Components in Ophiopogonis Radix in the Process of Development
WU Fa-ming1, CAI Xiao-yang1,CHEN Hui1,BAO Xiao-hong2,LI Min1*,ZHOU Juan2
1.The Ministry of Education Key Laboratory of Standardization of Chinese Crudedrug, State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medicines Resources Co-founded by Sichuan Province and MOST, Chengdu University of TCM, Chengdu 611137, China; 2.Sichuan Institute for Food and Drug Control, Chengdu 611130, China
Abstract��OBJECTIVE To explore the accumulation rules of the effective constituents in Ophiopogonis Radix to provide theoretical support for its production and quality control. METHODS Ophiopogonis Radix samples in different growth phases were harvested, and the total polysaccharides, total flavones, and total saponins in the sampleswere measured by ultraviolet spectrophotometry. Methylophiopogonanone A, ophiopogonin C, ophiopogonin D, and ophiopogonin D�� were determined by HPLC. RESULTS The contents of total polysaccharides, total flavones, and total saponins in Ophiopogonis Radix were positively correlated with root growth time. The contents of three categories of constituents increased with the prolongation of root growth time, but the accumulative rate was inconsistent in different growth phases, which had a slight decrease after reaching the maximum at the end of March. The accumulation trend of six single components were basically the same to the three categories of constituents. For samples with same harvest time, the content of the main constituents was higher in the samples treated by paclobutrazol than those without paclobutrazol treatment. CONCLUSION The variation trend of the main chemical components in Ophiopogonis Radix is basically the same to that of the root growth. Methylophiopogonanone A and phiopogonin D are the most representative, and they could be taken as evaluation index to guide production and quality control of Ophiopogonis Radix.
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WU Fa-ming, CAI Xiao-yang,CHEN Hui,BAO Xiao-hong,LI Min,ZHOU Juan. Accumulation of Main Chemical Components in Ophiopogonis Radix in the Process of Development. Chinese Pharmaceutical Journal, 2016, 51(7): 533-537.
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2016, Vol. 51 
