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�й�ҩѧ��־ 2014, Vol. 49 Issue (13) :1121-1125    DOI: 10.11669/cpj.2014.13.007
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LI Xi-bin, YE Juan, ZHAO Lei*, XIA Peng-fei, PENG Xue-jing, YU Xiao-hui
Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Gansu College of Traditional Chinese Medicine, Lanzhou 730000,China

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Abstract�� OBJECTIVE To investigate the feasibility of cultivated and wild total secoiridoid glucoside in treating osteoarthritis (OA) by observing the effects of total secoiridoid glucoside on interleukin 1��(IL-1��) induced chondrocytes of rat in vitro. METHODS Take the four passage rat chondrocytes and divided into 4 groups: the chondrocytes were cultured with rats complete medium in group A, with 10 ng��mL-1 recombinant rat IL-1�� 24 h in group B, with 10 ng��mL-1 recombinant rat IL-1�� and cultivated total secoiridoid glucoside 24 h in group C (different concentrations of cultivated total secoiridoid glucoside is 50, 200, 400 ��g��mL-1), with 10 ng��mL-1 recombinant rat IL-1�� and wild total secoiridoid glucoside 24 h in group D(different concentrations of seed total secoiridoid glucoside is 50, 100, 200 ��g��mL-1).Immunocytochemistry and Elisa were performed to determine the expression levels of collagen type II and PGE2.RESULTS An MTT assay indicated that 50, 200, 400 ��g��mL-1 cultivated total secoiridoid glucoside showed no significant toxicity to chondrocytes(P>0.05) at 24 h; 50, 100, 200 ��g��mL-1 wild total secoiridoid glucoside showed no significant toxicity to chondrocytes(P>0.05) at 24 h. Immunocytochemistry showed that the expression of collagen Type��in group C and group D obviously increased compared with group B, but weak in group A, and positive expression gradually enhanced with the increase of concentration(P<0.05). ELISA results showed that the production of PGE2 in group C and group D significantly decreased compared with group B, but higher than that of group A, and with the increase of concentration, the lower the production of PGE2(P<0.05).CONCLUSION Cultivated and wild total secoiridoid glucoside can protect rat chondrocyte from OA induced by IL-1��.
Keywords�� total secoiridoid glucoside,   chondrocytes,   collagen type��,   PGE2,   Gentiana officinalis     
�ո�����: 2014-09-03;
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��ϣ��, Ҷ��, ����*�� .�ƹ���ܴ�����ѻ�ϩ�����ն԰�ϸ������-1���յ��´������ϸ����Ӱ��[J]  �й�ҩѧ��־, 2014,V49(13): 1121-1125
LI Xi-Bin, YE Juan, ZHAO Lei-* etc .Effect of Gentiana officinalis from Total Secoiridoid Glucoside on Interleukin-1��(IL-1��)-Induced Rat Chondrocytes[J]  Chinese Pharmaceutical Journal, 2014,V49(13): 1121-1125
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[1] JIN Y, ZHAO L, WU D H, et al.Cultivated Gentiana officinalis anti-inflammation and analgesia quality evaluation and its effective parts of research. Lishizhen Med Mater Med Res(ʱ���ҽ��ҩ), 2011,22(5):1145-1146. YU X H, ZHAO L, WANG J H, et al. The study of total secoiridoid glucoside in Gentiana officinalis H. Smith was treated by adsorption process with macroporous resin . Lishizhen Med Mater Med Res(ʱ���ҽ��ҩ), 2010,21(12):3179-3180. YU X H, ZHAO L, LI Y, et al. Identification and content determination of gentiopicroside in Gentiana officinalis H. Smith from different areas of Gansu. Chin J Mod Appl Pharm(�й��ִ�Ӧ��ҩѧ), 2010,27(6):504-507. Ch.P(2005) Vol I(�й�ҩ��2005����һ��) . 2005: 190; Appendix ��B. DARLING E M, ATHANASIOU K A. Rapid phenolypic changes in passaged articular chondrocyte subpopulation . J Orthop Res,2005,23(2):425-432. ZHANG Z G, ZHENG W P, SU K, et al. Isolation,culture and morphological characteristics of rabbit articular chondrocyte . J SUN Yat-Sen Univ(Med Sci Ed)(��ɽ��ѧѧ��:ҽѧ��), 2004, 25(1):63-66. MA C H, ZHANG T L. Clinical Orthopaedics(�ٴ�����ѧ). 2nd ed. Beijing: People��s Medical Publishing House, 2004:47-49. KRASNOKUTSKY S, SAMUELS J, ABRAMSON S B. Osteoarthritis in 2007. Bull NYU Hosp Jt Dis, 2007, 65(3): 222-228. EYRE D.Collagen of articular carticular.Arthritis Res,2002,4:30-35.
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