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�й�ҩѧ��־ 2014, Vol. 49 Issue (9) :736-740    DOI: 10.11669/cpj.2014.09.008
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JIA De-wu1, LUO Sheng-yong2, YE Shou-shan2, LI Rui2, JIA De-yun2,NI Guang-yu2, CHEN Zhi-wu3, ZHANG Guo-hui4, DAI Xiang-rong4
1.Affiliated Hospital of Anhui Medical University,Hefei 230032, China; 2.Anhui Provincial Institute of Medical Scince, Hefei 230061, China; 3.Dept of Pharmacology, Anhui Medical University, Hefei 230032; 4.Zhaoke Pharmaceutical(Hefei)Company Limited, Hefei 230088, China

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ժҪ Ŀ�� ̽�ֿ�ѪС����˨��(anti-platelet thrombolysin,APT)�Դ����������ȱѪ�ٹ�ע���˵ı������ü�����ơ����� SD����120ֻ,�����Ϊ�������顢ģ����(ȱѪ�ٹ�ע��)��������(�������� 1 mg��kg-1)��ѪС����˨�ظߡ��С��ͼ�����(0.02 ��0.01��0.005 mg��kg-1),ÿ��20ֻ��������˨������ȱѪ/�ٹ�עģ��,������Ϊѧ�����ֶ�����ѪС����˨�ضԾ�������ȱѪ�ٹ�ע�����񾭹��ָܻ���Ӱ��,̽��ѪС����˨�ض�Ѫ���г��������绯ø(SOD)���ԡ����׸��Ĺ�������ø(GSH-Px)���Լ�ȱѪ����֯�б���ȩ(MDA)��һ������(NO)������Ӱ��,TTCȾɫ�۲쿹ѪС����˨�ض��Թ��������Ӱ��,TUNEL��ԭλ��Ǻ���CA1����������Ԫϸ��,�۲������Ԫϸ��������Ӱ�졣��� ѪС����˨�ظߡ��м���������Ը���ȱѪ�ٹ�ע������񾭹����ϰ�֢״�����ٹ������;�ߡ��С��ͼ�������������ƻ��峬�������绯ø�����׸��Ĺ�����ø���ԵĽ���;�ߡ��м��������Լ�������֯�б���ȩ��һ�������ĺ���,����ȱѪ����ϸ���ĵ����� ���� ѪС����˨�ؾ��нϺõĿ���ȱѪ�ٹ�ע��������,����ƿ����뿹����������ϸ�������йء�
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Abstract�� OBJECTIVE To investigate the protective effect of anti-platelet thrombolysin(APT) on cerebral focal ischemia reperfusion injury induced by middle cerebral artery occlusion (MCAO) in rats. METHODS One hundred and twenty SD rats were randomly divided into six groups:sham group, model group, positive control group, APT 0.02 , 0.01, 0.005 mg�kg-1group. The rat focal ischemia-reperfusion injury model was induced by ligation of middle cerebral artery occlusion(MCAO). Neurobehavioral score was evaluated in each group; SOD and GSH-Px activities in blood serum were examined, MDA and NO contents in cerebral ischemia tissue were detected; the volume of cerebral infarction was evaluated by TTC staining; TUNEL staining was used to measure apoptosis. RESULTS Compared with model group, the APT( 0.02 , 0.01 mg�kg-1) group could relieve obviously the neurological deficit score and the size of infarct volume; the APT( 0.02 , 0.01, 0.005 mg�kg-1 ) group could increase SOD and GSH-Px activities; the APT( 0.02 , 0.01 mg�kg-1) group could decrease the content of MDA and NO, reduced significantly the number of TUNEL-positive neurons in the hippocampal CA1 region. CONCLUSION APT has protective effect on MCAO injury in rats, which maybe related to the antioxidation and the inhibition of apoptosis.
Keywords�� anti-platelet thrombolysin,   cerebral ischemia/reperfusion,   antioxidant,   apoptosis     
�ո�����: 2013-08-26;
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�ֵ���, ��ʤ��, Ҷ��ɽ�� .��ѪС����˨�ضԴ����������ȱѪ���˱������ü������[J]  �й�ҩѧ��־, 2014,V49(9): 736-740
JIA De-Wu-, LUO Sheng-Yong-, YE Shou-Shan- etc .Effect of Anti-Platelet Thrombolysin on Focal Cerebral Ischemia/Reperfusion Injury in Rats[J]  Chinese Pharmaceutical Journal, 2014,V49(9): 736-740
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