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�й�ҩѧ��־ 2011, Vol. 46 Issue (1) :57-60    DOI:
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LC-MS/MSͬʱ�ⶨ��Ѫ����5�ֿ�������ҩ���9�ֿ�����ҩ���Ũ��
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ZHANG Meng-qi1, JIA Jing-ying1, LU Xiao-pei2, LU Chuan1, CAO Wei-e3, LI Shui-jun1, LIU Gang-yi1, YU Chen1*
1.Central Laboratory, Shanghai Xuhui Central Hospital��Shanghai 200031��China��2.Department of Analytical Chemistry, Shanghai Clinical Research Center, Shanghai 200233��China��3.Department of Cardiology, Shanghai Xuhui Central Hospital��Shanghai 200031��China

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ժҪ Ŀ�� ����LC-MS/MS�ⶨ��Ѫ�����ȵ�ƽ������ऴ�����ɳƽ������ͪ��̩��������̪����������ɳ�����װ�ɫ�֡��׵�ƽ�����ȱ�����������͡������͡������ͪ����������ѪҩŨ�ȵ����� �� ���� Ѫ�徭����ֱ�ӳ������÷����Ƿ���Ϊ�ڱ���вⶨ��ɫ������XbridgeTM Phenyl (2.1 mm��150 mm��5 ��m��Waters��˾�������ࣺ0.02%����ˮ��Һ-0.02%����������Һ��60��40���ȶ�ϴ�ѣ�����0.3 mL�� min-1���������3 ��L�����������ӻ���������MRMɨ�衣��� �ȵ�ƽ����̪����������ɳ�����װ�ɫ�֡��׵�ƽ�����ȱ���������͡������ͪ�������������Է�Χ��Ϊ10~1 000 ng�� L-1��r>0.996���������޾�Ϊ10 ng�� L-1������ऴ�������ͪ��̩�������Է�Χ��Ϊ1~100 ng�� L-1��r>0.997���������޾�Ϊ1 ng�� L-1����ɳƽ��������͡���Է�Χ��Ϊ5~500 ng�� L-1��r>0.998���������޾�Ϊ5 ng�� L-1��ƽ����ȡ�����ʾ�>87%�����ڡ����侫�ܶ�RSD��<15%������ ������ �����ȸߡ�רһ�Ժá������򵥣�������5�ֿ�������ҩ���9�ֿ�����ҩ����о���
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Abstract�� OBJECTIVE To develop a LC-MS/MS method for the determination of clozapine�� haloperidol�� loxapine�� risperidone�� tiapride�� citalopram�� fluvoxamine�� mianserin�� mirtazapine�� moclobemide�� paroxetine�� reboxetine�� trazodone and venlafaxine in human serum. METHODS The serum samples were precipitated with acetonitrile. The internal standard was vardenafil. The analytical column was XbridgeTM Phenyl (2.1 mm��150 mm��5 ��m��Waters. The mobile phase consisted of 0.02% formic acid water -0.02% formic acid acetonitrile (60��40�� at the flow rate of 0.3 mL��min-1. The injection volume was 3 ��L. The protonated ions of analytes were detected in positive ionization by multiple reactions monitoring (MRM mode.RESULTS The linear ranges of clozapine�� citalopram�� fluvoxamine�� mianserin�� mirtazapine�� moclobemide�� reboxetine�� trazodone and venlafaxine were validated from 10 to 1 000 ng�� L-1 ��r>0.996in plasma, and the low limits of quantitation was 10 ng�� L-1. The linear ranges of haloperidol�� risperidone and tiapride were validated from 1 to 100 ng�� L-1 ��r>0.997in plasma, and the low limits of quantitation were 1 ng�� L-1. The linear ranges of loxapine and paroxetine were validated from 5 to 500 ng�� L-1 ��r>0.998in plasma and the low limits of quantitation were 5 ng��L-1. The extraction recovery for all analytes was above 87%. The intra- and inter-batch precisions (RSD were all less than 15%. CONCLUSION The established LC-MS/MS method is shown to be sensitive, accurate, simple and suitable for the research of five antipsychotics and nine antidepressants.
Keywords�� antipsychotics,   antidepressants,   LC-MS/MS,   serum     
�ո�����: 2011-11-11;
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.LC-MS/MSͬʱ�ⶨ��Ѫ����5�ֿ�������ҩ���9�ֿ�����ҩ���Ũ��[J]  �й�ҩѧ��־, 2011,V46(1): 57-60
.Simultaneous Determination of Five Antipsychotics and Nine Antidepressants in Human Serum by LC-MS/MS[J]  Chinese Pharmaceutical Journal, 2011,V46(1): 57-60
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[1] ʩ���� ���� ���ļ� ��ȫӢ .����������͡��Ĥ��Ƭ�������Ч���о�[J]. �й�ҩѧ��־, 2012,47(5): 367-370
[2] ����� �׷� ����ˬ Ԭ���� ¬ϣ ����� ��ˬ ������ ������.������ľ������Ѫ��׵��׼�Ѫ���ɷֽ�����о�[J]. �й�ҩѧ��־, 2012,47(3): 228-
[3] ���� ����� ����ɽ .��Ѫ��׵��׵��Ʊ���Ӧ���о���չ[J]. �й�ҩѧ��־, 2011,46(24): 1857-1859
[4] ����� �ſ� ������ ���ٻ� ����÷ ���� �ņ���.�ɲ���ע��Һ�ڽ����������е�ҩ������ѧ�о�[J]. �й�ҩѧ��־, 2011,46(20): 1597-1600
[5] ֣ѧ�� ���� � �ֵ��� ������.�᳦����ţѪ�嵰�׻���Ƭ�����ƺ���������[J]. �й�ҩѧ��־, 2011,46(1): 35-39
[6] ������ �Ϸ��� ���̷� ˾���� ����ޱ ������.����޽���ڴ������ڵ�ҩ��ѧ�о�[J]. �й�ҩѧ��־, 2010,45(7): 539-542
[7] ¥���� ���� �����.HPLC-MS�ⶨ��Ѫ���а�������Ũ�ȼ�ҩ��ѧ[J]. �й�ҩѧ��־, 2010,45(4): 292-294
[8] ��˼�� ��� ��ѧ�� ֣���� ��Ӣ��.�����Թ������߻��ʽ�������ø-9�����ı仯��������������Ӱ��[J]. �й�ҩѧ��־, 2010,45(4): 313-314
[9] ������ ����.ǰҩҶ��-ţѪ��׵���-�������ż�������������ڷֲ���ҩЧѧ�о�[J]. �й�ҩѧ��־, 2010,45(2): 107-110
[10] �Ŵ��� �챦Ӣ ������ ������ ���� ������.��ЧҺ��ɫ��-�������׷��ⶨ��Ѫ�����ȷ�������Ũ��[J]. �й�ҩѧ��־, 2010,45(19): 1492-1495
[11] ������ ������ ��ѩ � ��� �����.Σ��֢��Ⱦ����Ѫ����������Ũ�ȼ��[J]. �й�ҩѧ��־, 2010,45(16): 1255-1258
[12] �ַƷ� ��Ц�� ������ �Ӵ�� .Һ��ɫ��-�������׷��ⶨ��Ѫ���л�����ù��[J]. �й�ҩѧ��־, 2010,45(13): 1020-1023
[13] ���� ��̫�� ���� ���� ����÷ ������.HPLC-MS/MS�ⶨ��������չ���ѪҩŨ��[J]. �й�ҩѧ��־, 2010,45(10): 772-774
[14] ����� �Ὺ�� ��Ц�� ���ݷ� �Ӵ�� ���ϴ�.����ǰ�д�Ƭ��ͬ��ҩ;��������ҩ��ѧ�о�[J]. �й�ҩѧ��־, 2010,45(1): 52-55
[15] ë��� ۣ��ΰ ���� ���� ����.Һ��ɫ��-�������׷�ͬʱ�ⶨ��ҩ����74��ũҩ������[J]. �й�ҩѧ��־, 2010,45(1): 64-70
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