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�й�ҩѧ��־ 2011, Vol. 46 Issue (2) :89-92    DOI:
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򾼤ø�ԻѪС��[Ca2+]i��JNK1��P-ѡ���ر���Ӱ��
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BI Hui�� WANG Li-an�� JI Hong-rui�� WANG Yu-ping�� DU Zhi-min*

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ժҪ Ŀ�� �о�򾼤ø(lumbrokinase�� LK�Դ���ѪС���ڸ�����Ũ�ȣ�intracellular calcium concentration�� [Ca2+]i��c-Jun����ĩ�˼�ø-1 (c-Jun N-terminal kinase-1�� JNK1�Լ���ѪС��P-ѡ����(P-selection�� Ps���ˮƽ��Ӱ�죬����LK��ѪС���IJ��ֻ��ơ����� ��ӫ��ֹ��ȼƲⶨLK�Դ���ѪС�������а���[Ca2+]i��Ӱ�죻���ߵ���ӡ�Ƿ������LK�Դ���ѪС��JNK1���ữˮƽ��Ӱ�죻��ʽϸ���DzⶨLK����ѪС��������Ps���ˮƽ��Ӱ�졣��� LK�߼����飨100 mg��L-1���м����飨50 mg��L-1����ѪС��[Ca2+]i�ֱ�Ϊ��425.49��37.4 nmol��L-1�ͣ�509.65��35.67 nmol��L-1�����յ���ѪС��[Ca2+]i ��555.59��39.71 nmol��L-1����������ͣ�P<0.01��P<0.05��LK�߼����飨100 mg��L-1���м����飨50 mg��L-1����ѪС��JNK1���ữˮƽ�ֱ�Ϊ��0.60��0.069�ͣ�0.79��0.048�����յ���ѪС��JNK1���ữˮƽ��0.87��0.037����������ͣ�P<0.01��P<0.05��LK�߼����飨100 mg��L-1���м����飨50 mg��L-1��ѪС��PsˮƽΪ��42.99��2.69�ͣ�44.72��2.09�����յ��飨49.99��3.81����������ͣ�P<0.01��P<0.05������ ����ʵ�����LK�ܹ����ƴ���ѪС�����[Ca2+]i���ߺ�JNK1�����ữ��ͬʱҲ��������ѪС��Ps�ı��ˮƽ�����ߣ��Ӷ��𵽿�ѪС�����á�
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Abstract�� OBJECTIVE To investigate the effect of lumbrokinase (LK on the level of intracellular free calcium�� concentration [Ca2+]i�� c-Jun N-terminal kinase-1��JNK1 and P-selection��Psin platelet�� moreover the underlying mechanisms of antiplatelet activation were elucidated. METHODS The spectrofluorimeter and western blotting were used for measuring thrombin-induced intracellular free calcium and JNK1 in rat platelet. In addition�� flow cytometry was employed to measure the thrombin-induced Ps in human platelet in vitro was determined by flow cytometr. RESULTS [Ca2+]i of high-dose group and medium-dose group were ��425.49��37.4 nmol��L-1 and ��509.65��35.67 nmol��L-1�� respectively�� and were significantly lower than the thrombin-induced group with rat platelet�� calcium concentration of ��555.59��39.71 nmol��L-1��P<0.01�� P<0.05; The level of P-JNK1 of high-dose group and medium-dose group were ��0.60��0.069 and ��0.79��0.048�� respectively and were significantly lower than the thrombin-induced group in rat platelet�� which was ��0.87��0.037 ��P<0.01�� P<0.05; The level of Ps of high-dose group and medium-dose group were ��42.99��2.69 and ��44.72��2.09�� and were�� significantly lower compared with the thrombin-induced group in human platelet�� which was ��49.99��3.81��P<0.01�� P<0.05. CONCLUSION LK suppress platelet activation through inhibiting [Ca2+]i and down-regulating the expression of JNK1 in rat platelet and reducing the level of Ps in human platelet in vitro.
Keywords�� lumbrokinase,   intracellular free calcium,   P-selection,   JNK1     
�ո�����: 2011-11-11;
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.򾼤ø�ԻѪС��[Ca2+]i��JNK1��P-ѡ���ر���Ӱ��[J]  �й�ҩѧ��־, 2011,V46(2): 89-92
.Effect of Lumbrokinase on [Ca2+]i�� JNK1 and P-Selection in Activated Platelet[J]  Chinese Pharmaceutical Journal, 2011,V46(2): 89-92
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