LI Wen-lan1��2��CHEN Qi1�� BAI Jing1��2��SUN Zhi1��YANG Yang1�� JI Yu-bin1��2
1.Research Center on Life Sciences and Environmental Sciences�� Harbin University of Commerce�� Harbin 150076�� China; 2.Engineering Research Ccenter of Natural Anticancer Drugs�� Ministry of Education�� Harbin 150076�� China
Abstract��
OBJECTIVE To identify the prototypes and metabolites of Bazhen Decoction in rat serum after oral administration. METHODS The chemical components of Bazhen Decoction in rat serum samples obtained after oral administration were analyzed by high performance liquid chromatography-electrospray ion trap mass spectrometry (HPLC-ESI-MS with selected ion monitoring and secondary fragment ion monitoring on line. RESULTS 22 Constituents�� including 16 prototypes and 6 metabolites�� were detected in serum samples. The prototypes were atractylenolide �� 5-hudroxymethyl-2-furfural�� hydroxypaeoniflorin�� atractylenolide �� albiflorin�� paeoniflorin�� liquiritin�� ferulic acid�� ligustilide�� ginsenoside Rg1 and ginsenoside Rb1. The metabolites were paeonimetabolin �� 3-O-��-D-18��-glycyrrhetic acid monoglucuronide�� glycyrrhetic acid�� ginsenoside Rh1 and ginsenoside Rd. CONCLUSION This sudy reveals the material base of the therapeutic effect of Bazhen Decoction�� and illustrates the principle of compatibility of traditional Chinese medicine. It also provides theoretic basis for establishing the quality standard of Bazhen Decoction.
2011, Vol. 46 
