LIU Pu1,2��ZHANG Chuang-feng1��DENG Rui-xue1��ZHAO Tian-zeng2 ��YIN Wei-ping1��2*
1.Chemical Engineering & pharmaceutical College��Henan University of Science and Technology��Luoyang 471003��China�� 2.Key Laboratory of Natural Products��Henan Academy of Sciences��Zhengzhou 450002��China
Abstract��
OBJECTIVE To establish an HPLC method for simultaneous determination of echinacoside��forsythoside B��verbascoside��isoverbascoside and oleuroprin in different parts of Syringa pubenscens. METHODS The separation was performed on an Agilent Zorbax SB C18 column (4.6 mm��250 mm��5 ��m)��using acetonitrile and potassium dihydrogen phosphate solution (pH 2.5) 20��80 as the mobile phase. The flow rate was 1.0 mL��min-1; the detection wavelengths were set at 334 nm (0-15 min) for echinacoside��forsythoside B��everbascoside and isoverbascoside��and 285 nm (15-25 min) for oleuroprin. The column temperature was set at 30 ��. RESULTS The linear ranges of echinacoside��forsythoside B��verbascoside��isoverbascoside and oleuroprin were 0.24��2.8 ��g (r=0.999 7)��0.32~1.6 ��g (r =0.999 9)��0.48~2.4 ��g (r=0.999 5)��0.36~1.8 ��g (r=0.999 8) and 0.4��3.6 (r=0.999 9)�� respectively. The average recoveries (n=3) were 99.19% with RSD of 0.41%��99.40% with RSD of 0.79%��99.45% with RSD of 76%��98.45%with RSD of 1.01%��99.5% with RSD of 0.73%��respectively.CONCLUSION The method is simple��accurate and can be used for quality control of Syringa pubenscens.
LIU Pu,
ZHANG Chuang-feng,
DENG Rui-xue etc
.Simultaneous Determination of Five Glycosides in Syringa pubenscens Turcz by HPLC[J] Chinese Pharmaceutical Journal, 2011,V46(24): 1935-1937
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2011, Vol. 46 
