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�й�ҩѧ��־ 2011, Vol. 46 Issue (24) :1935-1937    DOI:
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LIU Pu1,2��ZHANG Chuang-feng1��DENG Rui-xue1��ZHAO Tian-zeng2 ��YIN Wei-ping1��2*
1.Chemical Engineering & pharmaceutical College��Henan University of Science and Technology��Luoyang 471003��China�� 2.Key Laboratory of Natural Products��Henan Academy of Sciences��Zhengzhou 450002��China

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ժҪ Ŀ�� ����RP-HPLCͬʱ�ⶨСҶ���㲻ͬ��λ��5�������໯���ﺬ�������������� ����Agilent Zorbax SB C18ɫ����(4.6 mm��250 mm��5 ��m)��������Ϊ����-�������ػ�����(pH 2.5) 20��80�ȶ�ϴ�ѣ�����1 mL��min-1����Ⲩ��(�ɹ����գ���������B����Ҷ�����գ�����Ҷ������) 334 nm����魿���285 nm������30 �档��� �ɹ�������0.24��2.8 ��g����������B��0.32~1.6 ��g����Ҷ��������0.48~2.4 ��g������Ҷ������0.36~1.8 ��g����魿�����0.4��3.6 ��g�����������õ����Թ�ϵ��r�ֱ�Ϊ0.999 7��0.999 9��0.999 5��0.999 8��0.999 9��ƽ������������(n=3)�ֱ�Ϊ99.19%��99.40%��99.45%��98.45%��99.5%��RSDΪ0.41%��0.79%��0.76%��1.01%��0.73%������ ���������ټ�㡢�������ɿ���Ϊҩ��СҶ��������������ṩ�˿�ѧ���ݡ�
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Abstract�� OBJECTIVE To establish an HPLC method for simultaneous determination of echinacoside��forsythoside B��verbascoside��isoverbascoside and oleuroprin in different parts of Syringa pubenscens. METHODS The separation was performed on an Agilent Zorbax SB C18 column (4.6 mm��250 mm��5 ��m)��using acetonitrile and potassium dihydrogen phosphate solution (pH 2.5) 20��80 as the mobile phase. The flow rate was 1.0 mL��min-1; the detection wavelengths were set at 334 nm (0-15 min) for echinacoside��forsythoside B��everbascoside and isoverbascoside��and 285 nm (15-25 min) for oleuroprin. The column temperature was set at 30 ��. RESULTS The linear ranges of echinacoside��forsythoside B��verbascoside��isoverbascoside and oleuroprin were 0.24��2.8 ��g (r=0.999 7)��0.32~1.6 ��g (r =0.999 9)��0.48~2.4 ��g (r=0.999 5)��0.36~1.8 ��g (r=0.999 8) and 0.4��3.6 (r=0.999 9)�� respectively. The average recoveries (n=3) were 99.19% with RSD of 0.41%��99.40% with RSD of 0.79%��99.45% with RSD of 76%��98.45%with RSD of 1.01%��99.5% with RSD of 0.73%��respectively.CONCLUSION The method is simple��accurate and can be used for quality control of Syringa pubenscens.
Keywords�� Syringa pubenscens,   HPLC,   glycosides,   assay     
�ո�����: 2011-11-11;
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����1, 2, �Ŵ���1�� .��ЧҺ��ɫ�׷�ͬʱ�ⶨСҶ���㲻ͬ��λ��5�ֻ��Գɷֵĺ���[J]  �й�ҩѧ��־, 2011,V46(24): 1935-1937
LIU Pu, ZHANG Chuang-feng, DENG Rui-xue etc .Simultaneous Determination of Five Glycosides in Syringa pubenscens Turcz by HPLC[J]  Chinese Pharmaceutical Journal, 2011,V46(24): 1935-1937
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[1] Institute of Botany�� The Chinese Academy of Sciences. The Picture Index of Senior China Plant(�й��ߵ�ֲ��ͼ��) [M]. Beijing: Science Press�� 1983��350. [2] WU M J�� ZHAO T Z�� ZHANG H Y�� et al. Studies on chemical constituents of Syringa pubescens [J]. Chin Tradit Herb Drugs(�в�ҩ)�� 2003�� 34(1)�� 7-9. [3] WU M J�� ZHAO T Z�� ZHANG H Y�� et al. Studies on chemical constituents of Syringa pubescens [J]. Chin Tradit Herb Drugs(�в�ҩ)�� 2003�� 34(7)�� 594-595. [4] JING H�� ZUO J F�� LI J S. Development in research of phenylethanoid glycosides [J]. Lishizhen Med Mater Res (ʱ���ҽ��ҩ)�� 2006,17(3)��440-441. [5] ZHENG X K�� LIU Y Y�� FENG W S. Development in research of natural phenylethanoid glycosides[J]. Chin J New Drugs(�й���ҩ��־)�� 2011��20(3): 230-234. [6] BIISIGNOAO G�� TOMAINOA,CASCIO R L�� et al. On the in vitro antimicrobial activity of oleuropein and hydroxytyrosol [J]. J Pharm Pharmacol�� 1999�� 51��8����971. [7] DENG X D�� YUAN H�� LIU P�� et al. Chemical constituents from Syringa pubescens Turcz[J]. Biochem Syst Ecol�� 2010�� 38(9)��813-815. [8] DENG R X�� WANG Z�� DUAN W L�� et al. HPLC Determination oleuroprin in from different parts of Phlomis umbrosa[J]. Lishizhen Med Mater Res(ʱ���ҽ��ҩ)�� 2010�� 21(9)��2230-2231.
[1] ����ʤ ���� ������ ���� ��Ȼ ����.����ϳɹ�����Ī�ǿ���J�ĸ�ЧҺ��ɫ�׷��ⶨ����[J]. �й�ҩѧ��־, 2012,47(7): 543-546
[2] ���� ������ ���� ���� ŷ���� .�ǻ��컨��ɫ��A���컨��ȡ�P�����Ե���Ƭ���ǻ��컨��ɫ��A�ڴ������ڵ���й�Ƚ��о�[J]. �й�ҩѧ��־, 2012,47(6): 448-453
[3] ������ ��� ������ ������ �̱��� .��ЧҺ��ɫ�׷�ͬʱ�ⶨ�����䲻ͬ����Ʒ��5�ֽ����صĺ���[J]. �й�ҩѧ��־, 2012,47(6): 471-474
[4] ���� ������ ������ ������ .���ö�ָ��ɷ�����ȫ���齺��Ʒ�ʵ��о�[J]. �й�ҩѧ��־, 2012,47(5): 380-383
[5] ���� ���� ����� ��Ԫ�� ��·ƽ ������ .��é�з�����ɷָ�ЧҺ��ɫ�׷��ⶨ�����Ľ���[J]. �й�ҩѧ��־, 2012,47(5): 375-379
[6] ���� ʯ��� ���� .�������׷��ⶨ��������ע��Һ�ĺ���[J]. �й�ҩѧ��־, 2012,47(3): 232-
[7] ����Ƽ �ں��� ����� ����ϼ ���ۺ�.����������ƺ����ⶨ�ù��Ҷ���Ʒ������[J]. �й�ҩѧ��־, 2012,47(3): 236-
[8] ���� ����ƽ ֣ӱ.ͨ����ЧҺ��ɫ�ױ���ʱ��ⶨ����޽����-������Ԫ����ˮ����ϵ��[J]. �й�ҩѧ��־, 2012,47(2): 122-126
[9] ������ ����÷ ���� ������ ���ҷ� ʦ�پ�.ע����ͷ����̹�������й����������ڵ�ҩ��ѧ[J]. �й�ҩѧ��־, 2012,47(2): 132-135
[10] �׼��� ��Сƽ ���� Ѧ���� ����.��ЧҺ��ɫ�׷��ⶨ�Ѷ�������Ƥ�ء���ʶ��ء�ɽ����[J]. �й�ҩѧ��־, 2012,47(1): 69-71
[11] Ӧ��÷ ���н� ����ΰ ������ ���� �ᄚ��.HPLC-ELSD�ⶨϸ���������B������C�ĺ���[J]. �й�ҩѧ��־, 2011,46(9): 704-706
[12] ���� ���� ���ж� ����� ʩТ�� ������.��ŵ�������л���HPLC�ⶨ���ڴ���С���ܰ�ת���е�Ӧ��[J]. �й�ҩѧ��־, 2011,46(9): 686-690
[13] ���� �ņ��� ���ٻ� �ſ� ����� ������.������Ƭ�ڽ�������������Ч���о�[J]. �й�ҩѧ��־, 2011,46(9): 695-698
[14] � ���� ������ ����.��ҩ��ɽ�г�ɽ����쳣ɽ��ĺ����ⶨ[J]. �й�ҩѧ��־, 2011,46(8): 623-625
[15] ��Ⱥ ����ƽ �Ϸ� ��� ��ïֲ ����.�����ЧҺ��ɫ�׷��ⶨ��Ѫ��������ͷ����������[J]. �й�ҩѧ��־, 2011,46(7): 545-548
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