YE Xiao-min1��LI Hui-yi2��ZHANG Qi-ming2��FENG Fang3��TIAN Song-jiu2
1.Wuhan Institute for Drug Control��Wuhan 430012��China[2.National Institute for the Control of Pharmaceutical and Biological Products��Beijing 100050��China[3.China Pharmaceutical University��Nanjing 210009��China
Abstract��
OBJECTIVE To develop a headspace capillary gas chromatography method for the determination of residual organic solvents in porcine pulmonary surfactant.METHODS The samples were injected into HP-624 capillary column by headspace sampling and analyzed by using n-propanol as internal standard with FID detector.RESULTS The linear ranges of methanol, acetone and chloroform were 17.55��877.3 mg��L-1(r=0.999 9) ,29.62��1 481.8 mg��L-1(r=1.000 0)and 0.358 2-17.91 mg��L-1(r=0.999 8),respectively.The recoveries were 102.7%,98.1% and 99.3% with RSDs of 1.5% ,1.3% and 2.9% , respectively (n=9). CONCLUSION The method is simple, accurate and sensitive with good reproducibility.It can be used for the determination of the residual organic solvents in porcine pulmonary surfactant.
YE Xiao-min,
LI Hui-yi,
ZHANG Qi-ming etc
.Determination of Residual Organic Solvents in Porcine Pulmonary Surfactant by Capillary Gas Chromatography with Headspace Sampling [J] Chinese Pharmaceutical Journal, 2006,V41(01): 60-62
2006, Vol. 41 
