Abstract��
OBJECTIVE To investigate the anticonvulsion effect of Rana temporaria chensinensis David egg oil(EORTCD) and its relationship with monoamine neurotransmitters and their metabolites in spinal cord and brain of convulsion mice. METHODS The anticonvulsion effects, dose-response and time-efficacy relationship were measured by using the model induced by strychnine. Monoamine neurotransmitters and their metabolites in CNS,including norepinephrine (NA) , epinephrine (E) , dopamine (DA), 5-hydroxytryptamine (5-HT), Homovanil-lic acid (HVA) and 5-hydroxyindoleacetic acid(5-HIAA) ,were assayed by high performance liquid chromatography with electrochemical detector. RESULTS The anticonvulsion effect was reached dose-dependently at 30 min after EORTCD was administered. Strychnine decreased 5-HT, NA and HAV in spinal cord, accelerated the clearance of 5-HT and inhibited the transition of DA in spinal cord. Strychnine also inhibited DA transition in brain. EORTCD opposed the decrease, restored DA metabolic model and recovered 5-HT level to normal.CONCLUSION The inhibition action of EORTCD on CNS and the potent anticonvulsion effect on the model induced by strychnine are related to the monoamine neurotransmitters and their metabolites in spinal cord.
.Effects of Rana temporaria chensinensis egg oil on convulsion mice induced by strychnine nitrate and its relationship with monoamine neurotransmitters [J] Chinese Pharmaceutical Journal, 2005,V39(12): 912-915
2005, Vol. 39 