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�й�ҩѧ��־ 1998, Vol. 33 Issue (04) :227-229    DOI:
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Tang Ganghua
(Tang GH)��Jiang Guohui (Jiang GH)��Zhang Yun (Zhang Y)��et a

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ժҪ Ŀ�ģ������ⶨС��Ѫ��������������(NGF)Ũ�ȵķ����Ժ���(125I)ʾ�ٲⶨ����������125I-NGF�����Ȱ�T����ǣ�Ѫ����NGF��Ũ�Ȳ���ͬλ��ʾ�ٷ�������ȴ���(TCA)������SDS-PAGE��Ӿ���ⶨ�����Ƚ���ⶨ����������TCA������SDS-PAGE��Ӿ��ͬʱ���ղⶨ50��NGFѪ����Ʒ����������õ�����ԣ�r=0.9948�����ַ�������ͼ��Ũ�Ⱦ�Ϊ2.0ng��ml-1�����ڡ��ռ�RSD��С��10%,Ѫ����12.5ng��ml-1-400ng��ml-1Ũ�ȷ�Χ�ھ������õ������,���ϵ���ֱ�Ϊr=0.9990��r=0.9997��Ѫ����NGF��ƽ�������ʷֱ�Ϊ(95.21��3.70)%��(94.30��4.60)%�����ۣ�ͬλ��ʾ�ٷ���������Ӿ����ϣ������ȶ����ɿ��������Ƚϸߣ������ศ��ɣ�����NGFѪҩŨ�ȵIJⶨ��
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Abstract�� OBJECTIVE: To establish radioisotope (125I) tracer labelling methods for the determination of nerve growth factor (NGF) in mouse plasma. METHODS:125I��NGF was labelled with chloramine��T method, concentration of NGF in mouse plasma was determined by radioisotope tracer labelling method in conjunction with trichloroacetic acid (TCA) or SDS��PAGE, respectively, and the assay results of the two methods were compared. RESULTS: TCA method was used to measure the concentration of 50 mouse plasma samples compared with SDS��PAGE method. There was a good linear correlation (r=0.994 8) between the two methods. The lower limits of detection of the two methods for NGF in plasma were 2.0 ng��ml-1. Within��day and between��day coefficients of their variation were less than 10%. Their assay linearities were determined in the range of 12.5 ng��ml-1��400 ng��ml-1 in plasma (r=0.999 0 and r=0.999 7, respectively). The average recoveries of the two methods for NGF were 95.21% and 94.30%, respectively. CONCLUSION: Both the methods are constant, reliable and sensitive. They were complemental each other and are suitable for the determination of NGF concentration in mouse plasma.��
Keywords�� nerve growth factor,   isotope tracer labelling method,   TCA,   SDS��PAGE      
�ո�����: 1997-02-05;
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.ͬλ�ر��ʾ�ٷ��ⶨ����������ѪҩŨ��[J]  �й�ҩѧ��־, 1998,V33(04): 227-229
.Isotope tracer labelling method for determination of nerve growth factor plasma concentration [J]  Chinese Pharmaceutical Journal, 1998,V33(04): 227-229
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