1.Department of Pharmacy,302 hospital of PLA,Beijing 100039,China;2.Department of Pathology,302 hospital of PLA,Beijing 100039,China;3.Institute of Medicinal Biotechnology,Chinese Academy Medicinal Sciences and Peking Union Medical College,Beijing 100050,China
Abstract��
OBJECTIVE To study the inhibitory effect of Fuganneng(FNG) on hepatitic B virus(HBV).METHODS The 2.2.15 cell line,a human hepatoblastoma cell line transfected by HBV,was chosen as in vitro model and the HBV infected Beijing ducks were used as in vivo model.The level of HBsAg,HBeAg in 2.2.15 cell cultured medium and the DHBV-DNA (duck hepatitis B virus-DNA) in duckling serum were studied.RESULTS FGN could significantly inhibited HBsAg and HBeAg secreted by 2.2.15 cells at 8 d in vitro.FGN in high dose group had apparently inhibitory effect on HBsAg and HBeAg(P��0.01).FGN in high dose group and middle��dose group could significantly inhibited DHBV-DNA replication in vivo(P��0.05,P��0.01),and its inhibitory effect showed dose dependent and time��dependent.CONCLUSION FGN could significantly inhibit HBV,and showed its potential clinical application in HBV treatment.
2001, Vol. 36 
