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�й�ҩѧ��־ 2000, Vol. 35 Issue (06) :407-409    DOI:
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����ҽ�ƴ�ѧҩѧԺ���Ĵ� �ɶ� 610041����
YE Li-min,LI Zhang-wan,GUO Pin,HONG Zheng
School of Pharmacy,West China University of Medical Sciences,Chengdu 610041

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ժҪ Ŀ�� �������ȮѪ��ѪҩŨ��HPLC���л��ⶨ���������� Lichropred RP18(50 mm��4 mm��25��40 ��m)Ԥ��������ˮΪԤ���������ࡣShim-pack CLC-ODS(150 mm��6 mm��5 ��m)���������״�-ˮ(22��78)Ϊ���������ࡣ�����Ⲩ��270 nm����� ���Ѫ����ͼ��Ũ��Ϊ0.01 ��g��ml-1�����Է�Χ1��32 ��g��ml-1��ƽ������������Ϊ(95.8��1.33)%������ ���ڲⶨ12ֻ�й���������Ȯ�������Ƭ��200 mg��ѪҩŨ�ȣ�������á���
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Abstract�� OBJECTIVE High performance liquid chromatography with column switching was developed for the determination of theophylline in plasma of dog. METHODS The plasma samples were injected onto a pretreatment column packed with Lichromprep the RP 18(25��40 ��m)after simple dilution.Distilled water was used to wash out protein and other impurities in plasma.After switching,the concentrated theophylline was eluted in the back-flush mode onto a Shimpack CLC-ODS column with methanol-water (22��78)as mobile phase.Purge solutions were used for clean-up and for regenerating the pretreatment column. RESULTS The limit of detection was 0.001 mg��L-1plasma.The linear range was 1��32 mg��L-1.The ��RSD�� of intra-and inter-day were less than 3.37% and 5.36% for theophylline in dog plasma,respectively. CONCLUSION The method was successfully used to determine the pharmacokinetics of theophylline sustained release tablets in 12 dogs.
Keywords�� theophylline,   HPLC,   column switching     
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.��ЧҺ��ɫ�����л����ⶨȮѪ���в��[J]  �й�ҩѧ��־, 2000,V35(06): 407-409
.Determination of theophylline in dog plasma by high performance liquid chromatography with column switching[J]  Chinese Pharmaceutical Journal, 2000,V35(06): 407-409
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