Abstract��
OBJECTIVE High performance liquid chromatography with column switching was developed for the determination of theophylline in plasma of dog. METHODS The plasma samples were injected onto a pretreatment column packed with Lichromprep the RP 18(25��40 ��m)after simple dilution.Distilled water was used to wash out protein and other impurities in plasma.After switching,the concentrated theophylline was eluted in the back-flush mode onto a Shimpack CLC-ODS column with methanol-water (22��78)as mobile phase.Purge solutions were used for clean-up and for regenerating the pretreatment column. RESULTS The limit of detection was 0.001 mg��L-1plasma.The linear range was 1��32 mg��L-1.The ��RSD�� of intra-and inter-day were less than 3.37% and 5.36% for theophylline in dog plasma,respectively. CONCLUSION The method was successfully used to determine the pharmacokinetics of theophylline sustained release tablets in 12 dogs.
.Determination of theophylline in dog plasma by high performance liquid chromatography with column switching[J] Chinese Pharmaceutical Journal, 2000,V35(06): 407-409
2000, Vol. 35 
