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�й�ҩѧ��־ 2000, Vol. 35 Issue (05) :338-339    DOI:
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�������ָ�ע��Һ�������ƺ�����RP-HPLC�ⶨ��
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Zhu Zhao en
(Zhu ZE),Wang Hailong (Wang HL),Meng Minjie (Meng MJ

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ժҪ Ŀ�ģ������������ָ�ע��Һ�������ƺ�����RP-HPLC�ⶨ����������������UVɨ�跨�������ЧҺ��ɫ�׷�����YWG-C18Ϊ������������Һ(ȡע�����Ȼ���6.0 g��ע�����Ȼ���0.3 g��ע�����Ȼ���0.2 g��ˮ��1000 ml)Ϊ�����࣬ʹ����귨�������������������ղ���Ϊ(206��1) nm�������Է�Χ0.4516��5.645 g��L-1��r=0.9999����Ũ��ƽ�����������ʡ�RSD�ֱ�Ϊ(101.4��1.51)%��(100.5��0.81)%(n=6)�����ۣ�������㡢���١�׼ȷ������Ϊ��Ʒ�ļ�ⷽ������
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Abstract�� OBJECTIVE:To establish an RP��HPLC assay to determine sodium lactate contained in Ringer��s injection. METHODS:A UV scan method was applied.The RP��HPLC was conducted on a YWG C18 column (10 ��m,4.6 mm��20 cm) with basic solution (mix6.0 g sodium chloride and 0.3 g potassium chloride and 0.2 g calcium chloride,then added water to 1000 ml) as mobile phase.It was used the external standard method. RESULTS:The maximal absorption wavelength of sodium lactated was detected at (206��1) nm.The peak area and concentration showed good correlation in the range of 0.4516��5.645 g��L-1,r=0.9999.The average recoveries and the relative standard deviations were obtained at two different concentration levels as (101.4��1.5)% and (100.5��0.81)% (n=6),respectively. CONCLUSION:The method was found to be bimple,quick and accurate for the determination of sodium lactate contained in lactated Ringer s injection.
Keywords�� lactated Ringer s injection,   lactate,   RP-HPLC     
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.�������ָ�ע��Һ�������ƺ�����RP-HPLC�ⶨ��[J]  �й�ҩѧ��־, 2000,V35(05): 338-339
.Determination of sodium lactate in lactated Ringer��s injection by RP-HPLC[J]  Chinese Pharmaceutical Journal, 2000,V35(05): 338-339
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