CHEN Yun-long1,2, HE Guo-qing1,HUA Yun-fen2,ZHANG Ming2
1.College of Life Science,Zhejiang University,Hangzhou 310012,China;2.College of Biosystem Engineering and Food Science,Zhejiang University,Hangzhou 310029,China
Abstract��
OBJECTIVE To isolate the water��soluble polysaccharides from ��Dendrobium moniliforme(L.) Sw. and investigate its structural feature. METHODS The polysaccharides prepared from Dendrobium moniliforme were purified with column chromatography on DEAE-cellulose,Sephacryl S-400 HR and Sephacryl S-200 HR.The structural feature of the main polysaccharide was elucidated by periodate oxidation,HPGPC,TLC,GC,GC-MS and IR respeitively. RESULTS Eight polysaccharides,DMP1a-1,DMP1a��2,DMP2a-1,DMP3a-1,DMP4a-1,DMP5a-1,DMP6a-1 and DMP7a-1,were obtained from Dendrobium moniliforme .The results indicated that DMP1a-1 was a glucomannan with a 1��4.798 glucose mannose ratio and Mr was 28 000.Periodate oxidation analysis indicated DMP1a-1 containing ��(1��3,1��4,1��6) glycosidic bond,appeared in the molar ratio of 8.75��23.59��1. CONCLUSION Eight polysaccharides are isolated from this medicinal plant for the first time.DMP1a-1 is a ��-D-pyranyl glucomannan.
CHEN Yun-long,
HE Guo-qing,
HUA Yun-fen etc
.Extraction isolation and characterization of polysaccharide from Dendrobium moniliforme[J] Chinese Pharmaceutical Journal, 2003,V38(07): 494-497
2003, Vol. 38 
