Abstract��
OBJECTIVE To study the protective effect of minocycline on beta-amyloid protein 25-35(A��25-35)-induced toxicity in cultured rat cortical neurons��METHOD S The toxicity model was established by treated neurons with A��25-35 in cultured rat cortical neurons��Cell death was determined by the release of cellular lactate dehydrogenase (LDH). Apoptosis was evaluated by Hoechest 33258 staining and DNA gel electrophoresis��The activity of caspase3 was assayed by fluorescent substrat��RESULTS Minocycline reduced the level of LDH in the medium of the neurons treated with A��25-35 and kept the survival of neurons��A��25-35 induced nuclic condensation and DNA fragment in neurons at 24 h.These effects were attenuated by minocycline��In addition��the activity of caspase3 was increased in neurons after treated with A��25-35. The activity of caspase3 was inhibited��CONCLUSION Minocycline inhibited A��25-35-induced apoptosis in cultured cortical neurons��The effects of minocycline might be through inhibiting the activation of caspase3 induced by A��25-35.
2003, Vol. 38 
