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�й�ҩѧ��־ 2008, Vol. 43 Issue (13) :1022-1024    DOI:
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LC-MS/MS�ⶨ����Ѫ�������߿���Ũ��
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1.�㽭��ѧҽѧԺ������һҽԺ�ٴ�ҩѧ�о����� ���� 310003;2.�㽭��ѧҩѧԺҩ�����������о��� ���� 310058
ZHOU Hui-li1��WU Guo-lan2�� SHENTU Jian-zhong1*�� HU Xing-jiang1��ZHU Di-feng2��HE Qiao-jun2
1.Institute of Pharmacology & Toxicology and Biochemical Pharmaceutics��College of Pharmaceutical Sciences��Zhejiang University��Hangzhou 310058�� China��2.Department of Clinical Pharmacology��First Afiliated Hospital��College of Medicine��Zhejiang University��Hangzhou 310003�� China

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ժҪ Ŀ�Ľ����ⶨ����Ѫ�����߿���Ũ�ȵ�Һ�മ������(LC-MS/MS)��������Ѫ����Ʒ�õ��׳�������Ԥ������ZORBAX SB-C18(3.0 mm��150 mm,3.5��m)ɫ����Ϊ������,������:�״�-ˮ(80:20,����5 mmol��L-1�����),����:0.4 mL��min-1,ͨ������������Դ(ESI)�Զ��ط�Ӧѡ�����Ӽ��(MRM)��������ģʽ�����߿����ڱ겼�ȿ���ĸ���Ӽ�������m/z�ֱ�Ϊ275.3/126.2,289.4/140.3��������߿�����6.58��6 580��g��L-1����������,��������ͼ��Ũ��Ϊ6.58��g��L-1,������������93.29%��98.32%֮��,���ڡ��ռ侫�ܶ�(RSD)��С��6.92%�����۴˷������١�׼ȷ������,������ҩ��ѧ�о���
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Abstract�� OBJECTIVE To develop a sensitive and selective LC-MS/MS method for the determination of ropivacaine in guinea pig serum.METHODS After a simple sample preparation was procedure by one-step protein precipitation with acetonitrile, ropivacaine and the internal standard bupivacaine were separated on a Zorbax SB-C18(3.0 mm��150 mm,3.5��m) column.The mobile phase consisted of methanol-water(80:20,containing 5 mmol��L-1 ammonium formate) at a flow rate of 0.4 mL��min-1. Detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring(MRM)mode via eletrospray ionization(ESI)source.In positive mode,ropivacaine and internal standard bupivacaine produced precursor to product ion combinations at m/z 275.3/126.2 and 289.4/140.3,respectively.RESULTS The calibration curve was demonstrated to be linear over the concentration range of 6.58��6 580��g��L-1.The method had a lowest limit of quantification of 6.58��g��L-1.The relative recoveries were 93.29%��98.32%.The intra-day and inter-day precisions(RSD) were below 6.92%.CONCLUSION The method was proved to be convenient,sensitive,rapid and suitable for pharmacokinetics study.
Keywords�� ropivacaine,   LC-MS/MS,   pharmacokinetics      
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.LC-MS/MS�ⶨ����Ѫ�������߿���Ũ��[J]  �й�ҩѧ��־, 2008,V43(13): 1022-1024
.Determination of Ropivacaine in Guinea Pig Serum by Liquid Chromatography-Tandem Mass Spectrometry [J]  Chinese Pharmaceutical Journal, 2008,V43(13): 1022-1024
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