Quality Evaluation of Forsythiae Fructus from Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis
WEI Li-fang, MEI Yu-qi, ZOU Li-si, CHEN Jia-li, TAN Meng-xia, WANG Cheng-cheng, CAI Zhi-chen, LIN Li-qun, LIU Xun-hong*
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Abstract：OBJECTIVE To comprehensively evaluate the quality of Forsythiae Fructus from different habitats based on simultaneous determination of multiple active components combined with multivariate statistical analysis. METHODS Twenty-one bioactive constituents including phenylethanoid glycosides, lignans, flavonoids and phenolic acids in Forsythiae Fructus from different producing areas were simultaneously determined by ultra-fast liquid chromatography coupled with triple quadrupole/linear ion trap tandem mass spectrometry (UFLC-QTRAP-MS/MS). Based on the contents of 21 target components, hierarchical cluster analysis (HCA), principal component analysis (PCA) and One-way analysis of variance (One-way ANOVA) were further employed for the quality assessment of Forsythiae Fructus from different habitats. RESULTS The HCA results showed that 14 batches of medicinal materials from three major producing areas were divided into two categories: the first category was Lingchuan in Shanxi, Heyang in Shaanxi, Huixian in Henan, Linchuan in Henan and Luoyang in Henan; the second category was Anze in Shanxi and Guxian in Shanxi. The PCA results revealed that the comprehensive quality of Forsythiae Fructus from Shanxi was better than those from Shaanxi and Henan. Additionally, the results of One-way ANOVA indicated that the contents of 10 components, ie chlorogenic acid, ferulic acid, astragalin, arctiin, luteolin, forsythiaside B, forsythiaside A, pinoresinol-4-O-β-D-glucoside, phillyrin and pinoresinol were significantly different among the above three provinces. CONCLUSION The integrated evaluation system based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis is successfully applied to the quality evaluation of Forsythiae Fructus from different habitats. It can provide a new reference method for the quality evaluation and control of Forsythiae Fructus, which can also provide basic information for further expounding the geo-authentic of Forsythiae Fructus.
魏丽芳, 梅余琪, 邹立思, 陈佳丽, 谈梦霞, 王程成, 蔡芷辰, 林丽群, 刘训红. 基于多元活性成分同时测定结合多元统计分析评价不同产地连翘药材的质量[J]. 中国药学杂志, 2021, 56(4): 276-284.
WEI Li-fang, MEI Yu-qi, ZOU Li-si, CHEN Jia-li, TAN Meng-xia, WANG Cheng-cheng, CAI Zhi-chen, LIN Li-qun, LIU Xun-hong. Quality Evaluation of Forsythiae Fructus from Different Habitats Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis. Chinese Pharmaceutical Journal, 2021, 56(4): 276-284.
WANG Z Y, XIA Q, LIU X, et al. Phytochemistry, pharmacology, quality control and future research of Forsythia suspensa (Thunb.) Vahl: a review[J]. J Ethnopharmacol, 2018, 210: 318-339.
[3]
GUO H, LIU A H, LI L, et al. Simultaneous determination of 12 major constituents in Forsythia suspensa by high performance liquid chromatography—DAD method[J]. J Pharm Biomed Anal, 2007, 43(3): 1000-1006.
[4]
GUO D D, ZHANG L, ZHU X F. Investigation report on the germplasm resources of Forsythiae Fructus[J]. Lishizhen Med Mater Med Res (时珍国医国药), 2012, 23(10): 2601-2603.
[5]
WU T, WEI S, MI L H, et al. DNA fingerprint library construction and cluster analysis of Forsythia suspensa from different origins[J]. Chin Tradit Herb Drugs (中草药), 2016, 47(5): 816-820.
[6]
WU Y, WANG H, FANG M F, et al. Review of studies on influence of environmental factors on formation of medicinal materials from botanicals[J]. Nat Prod Res Dev (天然产物研究与开发), 2013, 25(3): 416-420.
[7]
NI Y N, ZHUANG H, KOKOT S. A high performance liquid chromatography and electrospray ionization mass spectrometry method for the analysis of the natural medicine, Forsythia Suspensa[J]. Anal Lett, 2014, 47(1): 102-116.
[8]
FU Y F, LI Q, BI K S. Determination of seven components in Forsythia suspensa by RP-HPLC[J]. Chin Tradit Herb Drugs (中草药), 2013, 44(8): 1043-1046.
[9]
FENG S. Quality evaluation study and variety and quality related research of Forsythiae Fructus based on chemical and biological fingerprint technology [D]. Jinan: Shandong University of Traditional Chinese Medicine, 2014.
[10]
WANG S N, HUA Y J, XU L, et al. Simultaneous determination of multiple constituents in Scrophulariae Radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS[J]. Chin Pharm J (中国药学杂志), 2016, 51(16): 1416-1422.
[11]
LI P H, WU Q N, YAN H, et al. Drying processing method for Zingiberis Rhizoma based on multiple bioactive constituents[J]. Chin Tradit Herb Drugs (中草药), 2018, 49(18): 4293-4301.
[12]
JIANG H, DING H P, BAI H J. Comprehensive assessment of quality characteristics of introduced table jujube cultivars in Southern Xinjiang[J]. Food Sci (食品科学), 2016, 37(3): 55-59.
[13]
DAN H L, MENG J. Variable selection for complex industrial control system based on principal component analysis[J]. J Chin Ceram Soc (硅酸盐学报), 2002, 30(S1): 131-134.
[14]
TAN M X, CHEN J L, ZOU L S, et al. Effects of different drying methods on multiple bioactive constituents of Ophiopogonis Radix[J]. Nat Prod Res Dev (天然产物研究与开发), 2019, 31(1): 100-109.
[15]
WANG Z. Analytical method and strategy for discovery, identification and determination of target multiple compounds in complex matrix samples [D]. Beijing: Peking Union Medical College, 2016.
[16]
HU M Z, MENG X S, WANG C, et al. Advances in application of mass spectrometry in high-throughput and rapid detection[J]. J Instrum Anal (分析测试学报), 2018, 37(2): 127-138.
[17]
PENG L, WANG Y Z, ZHU H B, et al. Fingerprint profile of active components for Artemisia selengensis Turcz by HPLC-PAD combined with chemometrics[J]. Food Chem, 2011, 125(3): 1064-1071.
2021, Vol. 56 
