Determination of 14 Bile Acids in Human Plasma by LC-MS/MS and Its Application in Research of Hepatotoxicity Induced by Cyclosporine A
LI Xiang-lian1,2, MI Yi-jun1,3, LIU Lin-sheng1, MIAO Li-yan1,2*
1. Department of Clinical Pharmacology Research Lab, The First Affiliated Hospital of Soochow University, Suzhou 215006, China; 2. College of Pharmaceutical Sciences, Soochow University, Suzhou 215100, China; 3. Center for Drug Adverse Reaction Monitoring of Wuxi, Wuxi 214029, China
Abstract：OBJECTIVE To establish a method for simultaneous quantitative determination of 14 bile acids in human plasma by liquid chromatograohy-tandem mass spectrometry (LC-MS/MS) and to explore the correlation between the hepatotoxicity induced by cyclosporine A and bile acids. METHODS Plasma samples were extracted by protein precipitation and bile acids were separated on a Waters ACQUITY UPLC HSS T3(2.1 mm×150 mm,1.8 μm) column with a flow rate of 0.3 mL·min-1. The mobile phase was water (containing 4 mmol·L-1 ammonium acetate) and 95% acetonitrile using gradient elution in 15 min. The detection system used an electrospray ion source in negative ion mode. RESULTS Fourteen bile acids had a good linear relationship, the intra-and inter-assay RSD values were less than 15%, the accuracy was between 87% and 116%, and the extraction recovery rates of the quality was between 101% and 120%. The concentration of cholic acid (CA) was well correlated with cyclosporine A and suggested that bile acids may be associated with the hepatotoxicity induced by cyclosporine A. CONCLUSION This LC-MS/MS method for quantitatively determining various bile acids in human plasma is convenient, accurate and sensitive and can be used for further basic research in hepatotoxicity caused by cyclosporine A.
李香莲,糜怡珺,刘林生,缪丽燕. LC-MS/MS同时测定人血浆中14种胆汁酸及其在环孢素肝毒性研究中的应用[J]. 中国药学杂志, 2020, 55(3): 213-217.
LI Xiang-lian, MI Yi-jun, LIU Lin-sheng, MIAO Li-yan. Determination of 14 Bile Acids in Human Plasma by LC-MS/MS and Its Application in Research of Hepatotoxicity Induced by Cyclosporine A. Chinese Pharmaceutical Journal, 2020, 55(3): 213-217.
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2020, Vol. 55 
