贵州医科大学, a. 贵州省药物制剂重点实验室,省部共建药用植物功效与利用国家重点实验室;b. 民族药与中药开发应用教育部工程研究中心;c. 药学院, 贵阳 550004
Investigation of the Excretion of Seven Constituents of Inula cappaort in Rat Urine by UPLC-MS/MS
LI Naa,c, YANG Shu-tinga,c, LU Yuana, GONG Zi-penga, LI Yong-junb, CAO Chuangc, WANG Yong-lina*
a. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics;b. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Ministry of Education;c. School of Pharmacy, Guizhou Medical University, Guiyang 550004, China
Abstract：OBJECTIVE To establish an UPLC-MS/MS method for the analysis of seven compounds of Inula cappaort in rat urine to study their excretion. METHODS The urine samples in 0-2, 2-6, 6-12, 12-24, and 24-36 h were collected. Acquity UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) was used and the column temperature was set at 45 ℃, the mobile phase was 0.1% formic acid acetonitrile -0.1% formic acid aqueous solution in a gradient elution mode and flow rate was 0.25 mL·min-1. The detection was carried out by a triple quadrupole linear ion trap mass spectrometer in positive and negative ion mode with an electrospray source. Multiple reactions monitoring (MRM) mode was employed. RESULTS The calibration curves showed good linearity, with correlation coefficients of greater than 0.991 0 for all of the analytes within the concentration ranges. The intra-day and inter-day precisions (RSD) were all less than 15%. The extraction recoveries of the seven components were more than 84.41%, without obvious matrix effect, which met the requirements for analysis. CONCLUSION The established method is simple, rapid, and sensitive. It can be applied in the excretion study of the seven components of Inula cappaort extract in rat urine. The urine excretion test showed that the prototype excretion rates are low in rats, and the cumulative excretion rates are all less than 5%.
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LI Na, YANG Shu-ting, LU Yuan, GONG Zi-peng, LI Yong-jun, CAO Chuang, WANG Yong-lin. Investigation of the Excretion of Seven Constituents of Inula cappaort in Rat Urine by UPLC-MS/MS. Chinese Pharmaceutical Journal, 2019, 54(21): 1797-1803.
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2019, Vol. 54 
