To analysis differencied gene expression between mesenchymal stem cells and hepatocyte-like transformed cells
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Objective To analysis of differential on gene expression profiles between rat bone marrow mesenchymal stem cells and hepatocyte like cells which were directional differentiated by gene expression microarray technology. Method To make the rat’s MSCs directional differentiation into hepatocyte-like cells this procedure was repeated 3 times, to amplified respectively MSCs and hepatocyte-like cell total RNA, fluorescent labeled Cy3 probe. And then basing on fluorescence in situ hybridization, scanning, screening the differential information with GO and pathway analysis. The results were validated by RT-PCR. Results The test results appeared to MSCs Vs HLCs 839 genes was different , among 448 genes up-regulated, 391 genes down –regulated,363 genes with biological process annotation, 377 genes with cellular components annotation, 364 genes with molecular function annotation. The Pathway analysis results revealed 45 pathways had significant changed among the 273 Pathways, 275 differentially expressed genes involved in those pathway, including 122 up-regulated genes and 153 down regulated genes. Constructing gene regulatory network through those differential gene expression, according to the degree in the network and selected 5 key gene: Pkm2, Nt5e, Fos, Adcy3, Itga7. Conclusion In this procedure that MSCs was induced and differentiate into HLCs in vitro, These regulate gene expression in transformed cells just as the same as liver cells, such as: proliferation, differentiation, apoptosis, metabolism and regulation are closely related.