Basic & Clinical Medicine ›› 2018, Vol. 38 ›› Issue (3): 330-334.
Previous Articles Next Articles
Received:
Revised:
Online:
Published:
Abstract: Objective: To investigate the effects of ER-α36 on invasion of human gastric cancer cell lines SGC7901 by miR-143. Methods: Lentiviral vectors were constructed to generate up-and down-regulations of miR-143 lentiviruses (LV-miR-143 and LV-anti-miR-143, respectively).The viruses were used to infect human gastric cancer cell lines SGC7901.The ER-α36 protein expression level and the invasion ability of constructed cells were detected by Western blotting and transwell. The target gene of miR-143 was predicted by bioinformatics tools. Luciferase reporter assay was carried out to confirm the predicted target gene. Results: The infection efficiency of the lentivirus titers of LV-miR-143 and LV-anti-miR-143 were over 80% by the observation of green fluorescence. The ER-α36 expression level, the cell invasion ability in LV-miR-21 group were significantly lower than those in LV-anti-miR-21 group(p<0.05). Conclusions: miR-143 plays an important role in the negative control of gastric cancer invasion by the regulation of ER-α36.
Key words: Gastric cancer, ER-α36, Cell invasion, miR-143
CLC Number:
R735.2
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2018/V38/I3/330