Resveratrol alleviates LPS-induced heart injury in mouse models

doi:10.16352/j.issn.1001-6325.2022.12.1867

Abstract

Abstract: Objective To investigate whether resveratrol (RES) can alleviate lipopolysaccharide (LPS)-induced heart damage in mice. Methods Mice were randomly divided into control group, LPS group(intraperitoneal LPS 7.5 mg/kg) and RES low(20 mg/kg) and high-dose(60 mg/kg) intervention groups. The cardiac function was examined by ultrosound method; heart tissue sections was microscopied with HE staining for morphological change and apoptosis of heart tissue; Western blot was used to examine the expression of the apoptosis-related proteins Bax and Bcl-2. Microplate assay was used to detect CK-MB and LDH in serum; ELISA was performed to detect the proinflammatory cytokines IL-1β, IL-6 and TNF-α levels in serum and a commercially available kit was used to detect SOD viability and MDA content in heart tissue homogenates. Results Compared with the control group, LPS group mice showed a decreased cardiac function(P＜0.05) and morphological change of tissue(P＜0.05), significant increase of CK-MB, LDH, IL-1, IL-6, TNF-α release(P＜0.05), decreased SOD activity(P＜0.05), increased MDA content(P＜0.05), increased myocardial apoptotic cells and Bax to Bcl-2 ratio(P＜0.05) were recorded. The RES intervention significantly reversed these changes (P＜0.05), with a dose-dependent manner. Conclusions RES alleviates LPS-induced cardiac damage through an anti-inflammatory,antioxidant and anti-apoptotic mechanism.

Key words: resveratrol, cardiac dysfunction, apoptosis, inflammation, oxidative stress

CLC Number:

R965.2

ZHENG Ling, CHEN Li, WANG Yue. Resveratrol alleviates LPS-induced heart injury in mouse models[J]. Basic & Clinical Medicine, 2022, 42(12): 1867-1872.

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URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/10.16352/j.issn.1001-6325.2022.12.1867

http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2022/V42/I12/1867

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