Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (11): 1667-1674.doi: 10.16352/j.issn.1001-6325.2022.11.1667

• Original Articles • Previous Articles     Next Articles

Silencing circ_0006104 inhibits the proliferation, activation and collagen synthesis by mouse cardiac fibroblasts through up-regulation of miR-370-3p

LI Ya-fei1, DU Ying-qiang1, WANG Ze-mu2, ZHANG Jun1*   

  1. 1. Department of Cardiology, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215000;
    2. Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000,China
  • Received:2021-10-08 Revised:2022-01-28 Online:2022-11-05 Published:2022-11-01
  • Contact: * zhangjun0808@njmu.edu.cn

Abstract: Objective To explore the function and mechanism of circular RNA 0006104 (circ_0006104) in regulating proliferation, activation and extracellular matrix (ECM) synthesis of cardiac fibroblasts (CFs) induced by angiotensin Ⅱ (Ang Ⅱ). Methods The primary CFs of neonatal mice were isolated and divided into control group, circ_0006104 knockdown group, Ang Ⅱ group,Ang Ⅱ + circ_0006104 knockdown group and Ang Ⅱ + circ_0006104 knockdown+ miR-370-3p inhibitor.EDU immunofluorescence staining was used to detect the proliferation level of CFs; Western blot was used to detect activation related gene (α-SMA); RT-qPCR was used to detect the expression of collagen Ⅰ and Ⅲ (Col and Col Ⅲ). Results Circ_0006104 was screened by RNA-seq high-throughput sequencing results. In the primary CFs of neonatal mice, silencing circ_0006104 had no effect on the proliferation, activation and ECM synthesis of CFs under physiological conditions, while silencing circ_0006104 could significantly inhibit the proliferation, activation and ECM synthesis of CFs induced by Ang Ⅱ (P<0.05). The molecules of circ_0006104 could bind to and negatively regulate miR-370-3p. The combined experimental results showed that inhibition of miR-370-3p significantly reversed the inhibitory effects of circ_0006104 silencing on the proliferation, activation and ECM synthesis of CFs induced by Ang Ⅱ (P<0.05). Conclusions Knockdown of circ_0006104 inhibits Ang Ⅱ-induced CFs fibrosis by binding and negatively regulating miR-370-3p.

Key words: cardiac remodeling, cardiac fibroblasts, circ_0006104, miR-370-3p

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