Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (6): 913-919.doi: 10.16352/j.issn.1001-6325.2022.06.022

• Original Articles • Previous Articles     Next Articles

Effect of lncRNA SNHG14 on oligomeric Aβ-induced injury in rat neural cell line PC12 through regulating miR-142-5p

SHEN Jie1, YAN Jie2, ZHONG Ming1, PANG Rui-juan1, LUO Yong-jie3*   

  1. 1. Department of Neurology; 2. Department of Laboratory, Chengdu Second People's Hospital, Chengdu 610017;
    3. Department of Neurology, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu 610072,China
  • Received:2021-10-28 Revised:2022-01-28 Online:2022-06-05 Published:2022-06-02
  • Contact: * m529mo@163.com

Abstract: Objective To investigate the effect of lncRNA SNHG14 targeting at miR-142-5p on the neuronal damage induced by oligomeric Aβ. Methods PC12 cells were divided into control group, model group, model+si-NC group, model+si-SNHG14 group, model+miR-NC group, model+miR-142-5p group, model+si-SNHG14+anti-miR-NC group, and model+ si-SNHG14+anti-miR-142-5p group. The level of SNHG14 and miR-142-5p was calculated by RT-qPCR. CCK-8 method and flow cytometry were applied to detect inhibition rate on cell proliferation and apoptosis rate. ELISA method was used to detect TNF-αand IL-6 in the culture. Dual luciferase report assay was used to find the targeting relationship between SNHG14 and miR-142-5p. Results SNHG14 was highly expressed (P<0.05)while miR-142-5p was lowly expressed (P<0.05) in plasma of AD patients. Compared with the control group, the SNHG14 level, cell proliferation inhibition rate, and apoptosis rate in the model group were significantly increased (P<0.05), the level of miR-142-5p was significantly reduced (P<0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly increased (P<0.05), IL-10 level was significantly decreased (P<0.05). Compared with the model+si-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+si-SNHG14 group were significantly reduced (P<0.05), the level of TNF-α and IL-6 in the culture medium was significantly reduced (P<0.05), IL-10 level was significantly increased(P<0.05). Compared with the model+miR-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+miR-142-5p group were significantly reduced (P<0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly reduced (P<0.05) and IL-10 level was significantly increased (P<0.05). miR-142-5p was a direct target of SNHG14. Compared with the model+si-SNHG14+ anti-miR-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+ si-SNHG14+anti-miR-142-5p group were significantly increased(P<0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly increased (P<0.05), IL-10 level was significantly decreased (P<0.05). Conclusions Silencing SNHG14 may alleviate oligomeric Aβ-induced neuronal damage by targeting at and promoting miR-142-5p expression.

Key words: Alzheimer's disease, SNHG14, miR-142-5p, oligomeric Aβ, nerve cell

CLC Number: