基础医学与临床 ›› 2010, Vol. 30 ›› Issue (3): 237-241.

• 研究论文 • 上一篇    下一篇

慢病毒PARG-shRNA转染降低大肠癌lovo细胞基质黏附、运动和侵袭能力

李巧转 王娅兰 李娴   

  1. 重庆医科大学病理学教研室 重庆医科大学
  • 收稿日期:2009-07-14 修回日期:2009-09-29 出版日期:2010-03-05 发布日期:2011-05-04
  • 通讯作者: 王娅兰

Lentivirus PARG-shRNA transfection decreases colon carcinoma lovo cells matrix adhesion, migration and invasion potencies

Qiao-zhuan LI, Ya-lan WANG, Xian LI   

  1. Chongqing Medical University Chongqing Medical University
  • Received:2009-07-14 Revised:2009-09-29 Online:2010-03-05 Published:2011-05-04
  • Contact: Ya-lan WANG,

摘要: 目的 探讨聚(腺苷二磷酸核糖)水解酶(PARG)基因沉默对人大肠癌lovo细胞基质黏附、运动和侵袭能力的影响。方法 慢病毒PARG-shRNA转染人大肠癌lovo细胞并筛选出稳定沉默PARG基因的lovo细胞株;Western blot法检测PARG,PARP和NF-κB的表达。用细胞基质黏附、运动和侵袭实验,观察lovo细胞基质黏附、运动和侵袭能力。结果 获得了稳定的PARG基因沉默lovo细胞株,实验组PARP和NF-κB的表达显著降低(p<0.05, p<0.05)。PARG沉默后lovo细胞黏附、运动和侵袭能力降低。和未转染组相比其黏附、运动和侵袭抑制率分别为25.22%、38.71%和35.29%。结论 PARG基因沉默可以降低lovo细胞基质黏附、运动和侵袭能力,此可能与PARG沉默后下调PARP,并下调NF-κB的活性有关。提示PARG在肿瘤侵袭转移中可能发挥重要作用。

关键词: PARG-shRNA, 基因沉默, 慢病毒, 大肠癌, 侵袭

Abstract: Objective To approach the effects of Poly(ADP-ribose) glycohydrolase (PARG) gene silencing on colon carcinoma lovo cells matrix adhesion, migration and invasion. Methods Lentivirus PARG-shRNA was transfected into colon carcinoma lovo cells and the lovo cell strain with PARG gene silencing perpetually was selected. Western blot was used to test the expression of PARG, Poly(ADP-ribose) polymerase(PARP) and NF-κB. The lovo cell matrix adhesion, migration and invasion potencies were observed by cell matrix adhesion, migration and invasion assay. Results We obtained lovo cell strain with PARG gene silencing perpetually. The expression of PARP and NF-κB in experiment groups was weaker than that in the control groups (p<0.05, p<0.05). PARG gene silencing of lovo cells decreased the cell matrix adhesion, migration and invasion potencies. The inhibitory rates of lovo cell matrix adhesion, migration and invasion were 25.22%、38.71%、35.29% separately. Conclusion The data suggested that PARG gene silencing of lovo cells could inhibite the cell matrix adhesion, migration and invasion potencies, it probably related that PARG gene silencing decreased the activity of PARP and NF-κB. PARG probably plays an important role in the infiltration and metastasis of tumour.

Key words: PARG-shRNA, gene silencing, lentivirus, colon carcinoma, invasion

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