基础医学与临床 ›› 2008, Vol. 28 ›› Issue (7): 719-721.

• 研究论文 • 上一篇    下一篇

束缚应激对大鼠血小板-氧化氮释放的影响

冯浩楼 崔玉英 魏芳 王保水 唐朝枢   

  1. 保定市河北大学医学部 保定市河北大学卫生职业技术学院 保定市河北大学卫生职业技术学院 保定市河北大学卫生职业技术学院
  • 收稿日期:2007-06-05 修回日期:2007-09-21 出版日期:2008-07-25 发布日期:2008-07-25
  • 通讯作者: 冯浩楼

Effect of water-immersion restraint stress on the nitric oxide production in rat platelets

Hao-lou FENG, Yu-ying CUI, Fang WEI, Bao-shui WANG, Chao-shu TANG   

  • Received:2007-06-05 Revised:2007-09-21 Online:2008-07-25 Published:2008-07-25
  • Contact: Hao-lou FENG,

摘要: 束缚应激对大鼠血小板NO释放的影响 冯浩楼1 , 崔玉英2* , 魏芳2 , 王保水2, 唐朝枢3 ( 1. 河北大学医学部; 2. 河北大学卫生职业技术学院 生理教研室, 保定 071000; 3.北京大学第一医院心血管研究所, 北京100034) 摘要:目的 观察不同时间的急性应激对大鼠血小板一氧化氮(NO)释放的影响及其机制。方法 大鼠浸水-束缚应激(water-immersion restraint stress,WRS) 2、4和8h,以胃溃疡指数(Ulcer Index, UI)作为机体应激损伤的标识,采用Greiss法测定血小板孵育液中亚硝酸盐(NO2-)含量;同位素法测其一氧化氮合酶(NOS)活性和L-精氨酸(L-Arg)转运量。结果 WRS 2h血小板L-Arg转运量、NOS活性和孵育液NO2-含量较对照组显著增加,但随应激时间延长,其呈下降趋势, 应激8h时均显著低于对照组,胃溃疡逐渐加重。结论 WRS应激早期阶段可上调血小板L-Arg/NO通路,促进血小板NO生成;长时间应激下调L-Arg/ NO通路,减少NO释放。

Abstract: Effect of water-immersion restraint stress on the nitric oxide production in rat platelets FENG Hao-lou1,CUI Yu-ying2, WEI Fang2, WANG Bao-shui 2,Tang Chao-shu3 ( 1. Dept. Physiology, Medical College, Hebei University; 2. Dept. Physiology, College of Vocational and Technological Sanitation, Hebei University, Baoding 071000, China; 3.Dept. Institute of Cardiovascular Research, Peking University First Hospital, Beijing 100034.) Abstract: Objective To investigate the effect of water-immersion restraint stress (WRS) on the nitric oxide (NO) production and its mechanism in rat platelets. Methods Male SD rats underwent WIR stress for 2, 4 and 8 hours. Ulcer index (UI) of stomach was detected by Guth method, nitrite production was measured by Greiss assay, nitric oxide synthase (NOS) activities and L-arginine transport rate were determined by isotope tracer method. Results After 2h WRS, the NO production, the NOS activity and L-arginine transport rate in rat platelets were obviously increased, but they tended to be gradually decreased as the time went on. After 8h of WRS, these parameters were markedly decreased compared with the control group. The gastric ulcer appeared and got more serious after 4h WIR stress. Conclusion Short-term WRS up-regulated L-arginine /NO pathway and increased nitric oxide production in the platelets while long-term WIR stress caused the reversed results.