基础医学与临床 ›› 2008, Vol. 28 ›› Issue (8): 816-819.

• 研究论文 • 上一篇    下一篇

大鼠全脑缺血再灌注后耳蜗组织内TrkA和NGF表达的改变

施宁华 张志坚 许燕 王东林 周志强   

  1. 江苏大学医学技术学院医学影像系 江苏大学 医学院 基础与临床医学研究中心 江苏大学医学院基础与临床医学研究中心 江苏大学 医学院 基础与临床医学研究中心 江苏大学医学院基础与临床医学研究中心 江苏大学 医学院 基础与临床医学研究中心 江苏大学医学院基础与临床医学研究中心
  • 收稿日期:2007-09-27 修回日期:2007-12-25 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 施宁华

Expression changes of TrkA and NGF in cochleas of rats after whole cerebral ischemia-reperfusion

Ning-hua SHI Zhi-jian ZHANG Yan XU Dong-lin WANG Zhi-qiang ZHOU   

  1. College of Medical Technology,Jiangsu University
  • Received:2007-09-27 Revised:2007-12-25 Online:2008-08-25 Published:2008-08-25
  • Contact: Ning-hua SHI

摘要: 目的 探讨大鼠全脑缺血再灌注后酪氨酸激酶A(TrkA)和神经生长因子(NGF)在耳蜗Corti器的表达。方法 建立大鼠全脑缺血再灌注模型,采用免疫组织化学染色方法检测TrkA和NGF在耳蜗中的表达;用TUNEL法检测耳蜗毛细胞发生凋亡的时相和分布。结果 再灌注后6h NGF表达增强,24h达高峰后逐渐回复,再灌注24h~7d TrkA表达减弱甚至不表达,3~7d期间耳蜗毛细胞出现凋亡,7~14d TrkA表达逐渐恢复。结论 TrkA的表达对于介导NGF促感觉神经细胞存活及损伤修复的生物效应有重要意义。

Abstract: Objective To study the expressions of tyrosine kinase A (TrkA) and nerve growth factor (NGF) on cochlear corti in rats after whole cerebral ischemia-reperfusion. Methods Establishing a model of whole cerebral ischemia-reperfusion in rats; examining the expressions of TrkA and NGF on cochleas assuming the method of immunohistochemistry stain; and examining the time and distribution of the cochlear hair cell apoptosis using the terminal deoxynucleotidyl transferasemediated dUTP nick end labeling (TUNEL). Results After ischemia-reperfusion the 6h NGF expression increased, the 24h NGF expression reached the highest and then restored gradually. After ischemia-reperfusion the 24h-7d TrkA expression weakened or even didn't expresse. During 3-7d the cochlear hair cells presented with apoptoses. During 7-14d TrkA expression restroed gradually. Conclusion TrkA expression is significant for mediating NGF to promote the sensory nerve cell surviving and for the biological effects of the repair of injury.