基础医学与临床 ›› 2008, Vol. 28 ›› Issue (5): 450-454.

• 研究论文 • 上一篇    下一篇

共刺激分子B7-H1在胃癌中表达上调

刘书漫 李倩如 张娓 王一菱 孟青 张钦宪 刘占举   

  1. 郑州大学基础医学院组胚教研室 郑州大学第二附属医院消化内科免疫病理实验室 郑州大学基础医学院组胚教研室 郑州大学基础医学院组胚教研室 郑州大学 基础医学院 郑州大学基础医学院组胚教研室 郑州大学第二附属医院消化内科免疫病理教研室
  • 收稿日期:2007-08-29 修回日期:2007-11-22 出版日期:2008-05-25 发布日期:2008-05-25
  • 通讯作者: 刘占举

Up-regulated expression of co-stimulatory molecule B7-H1 in gastric carcinoma

Shu-man LIU, Qian-ru LI, Wei ZHANG, Yi-ling WANG, Qing MENG, Zhan-ju LIU   

  1. School of Basic Medicine, Zhengzhou University School of Basic Medicine, Zhengzhou University Second Affiliated Hospital, Zhengzhou University
  • Received:2007-08-29 Revised:2007-11-22 Online:2008-05-25 Published:2008-05-25
  • Contact: Zhan-ju LIU

摘要: 目的 了解胃癌患者胃黏膜组织中共刺激分子B7-H1的表达情况及其与肿瘤转移和预后的关系。方法 应用流式细胞技术、免疫化学染色技术、免疫荧光染色技术与Western blot技术检测胃癌细胞系SGC-7901与新鲜切除的胃癌组织、癌旁组织及其远端正常胃黏膜组织中B7-H1的表达,并对相关数据进行相应的统计学分析,确定B7-H1表达水平与患者临床病理指标的关系。结果 SGC-7901细胞中有B7-H1蛋白表达,主要分布在细胞膜,细胞质中少量;胃癌组织中B7-H1阳性表达率为(13/21)62%,癌旁组织中为(7/21)33%,而正常胃黏膜组织中没有B7-H1表达;统计分析显示,胃癌组织中B7-H1表达与肿瘤的浸润深度、周围淋巴结转移、远处转移及pTNM分期有关( P< 0. 05)。结论 B7-H1分子可作为胃癌早期诊断与预后判断的新指标。

Abstract: Objective To study the expression of B7-H1 in gastric mucosa of patients with gastric carcinoma, and to identify its relationship with neoplasm metastasis and prognosis. Methods Expression of B7-H1 in gastric carcinoma cell line SGC-7901 and in freshly-resected gastric mucosa including gastric carcinoma, adjacent tumor tissue and distal normal gastric mucosa were determined by flow cytometric analysis, immunochemical staining, immunofluorescence staining and Western blot. Moreover, the correlated data was analyzed statistically. And so the correlation between the expression level of B7-H1 and the patients, clinicopathological parameters was established. Results B7-H1 expression was detected in SGC-7901 cell line. And B7-H1 was found to localize in cell membrane and little cytoplasm. The positive rate of B7-H1 expression in gastric carcinoma was (13/21)62%, and it was (7/21)33% in adjacent tumor tissue, Whereas B7-H1 was absent in distal normal gastric mucosa. The statistical analysis showed positive correlations between B7-H1 expression in gastric carcinoma and the depth of carcinoma infiltration, lymph node metastasis and pTNM stage (P < 0. 05). Conclusion B7-H1 molecule may be used as a new indicator to diagnose gastric carcinoma on early stage and to predict prognosis of this disease.