基础医学与临床 ›› 2008, Vol. 28 ›› Issue (8): 810-815.

• 研究论文 • 上一篇    下一篇

PPAR-γ偶联的信号通路可能参与大鼠肝再生

徐存栓 唐自阔   

  1. 河南师范大学生命科学学院 河南省科技部共建细胞分化调控重点实验室 河南省科技部共建细胞分化调控重点实验室
  • 收稿日期:2007-05-14 修回日期:2007-12-12 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 徐存栓

PPAR-γ coupled signaling pathways involved in rat liver regeneration

Cun-shuan XU Zi-kuo TANG   

  1. College of Life Sciences,Henan Normal University
  • Received:2007-05-14 Revised:2007-12-12 Online:2008-08-25 Published:2008-08-25
  • Contact: Cun-shuan XU

摘要: 目的 了解PPAR-γ偶联的信号转导通路在大鼠肝再生(LR)中作用。方法 用搜集网站资料和查阅相关论文等方法获得上述通路相关基因,用Rat Genome 230 2.0芯片检测它们在大鼠肝再生中表达情况,用真、假手术比较方法确定肝再生相关基因。结果 初步证实上述基因中64个基因与肝再生相关。肝再生启动、G0/G1过渡、细胞增殖、细胞分化和组织结构功能重建等四个阶段起始表达的基因数为28、4、34和2,基因总表达次数为72、41、247和90,表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共分为11种表达方式,表明肝再生中这些基因表达变化多样和复杂。结论 PPAR-γ偶联的信号转导通路在肝再生早期、前期和后期促进糖元合成;在整个肝再生中抑制炎症反应,促进细胞增殖和迁移。

Abstract: Objective PPAR-γ is acknowledgedly one of members of peroxisome proliferator activated receptors family, and acts by regulating a wide spectrum of biological activities such as carbohydrate metabolism, cell mobility, differentiation, apoptosis and so on. Of course, the above activities occur in liver regeneration. This study aims to investigate the possible contribution of PPAR-γ coupled signaling pathways to rat liver regeneration (LR) at transcription level. Methods The above signaling pathways-related genes were obtained by collecting database data and retrieving pertinent literatures. The gene expression changes during LR was checked by Rat Genome 230 2.0 array, and LR-associated genes were identified by comparing gene expression difference between partial hepatectomy (PH) and sham operation (SO) groups.Results 64 genes were found to be LR-associated. The number of initially and totally expressed genes occurring in initiation phase of LR, G0/G1 transition, cell proliferation, cell differentiation and structure-function reconstruction was 28, 4, 34, 2 and 72, 41, 247, 90 respectively, illustrating the related-genes were initially expressed mainly in initial phase and functioned in different phases. Classification of their expression patterns into 11 groups reflected diversity and complexity of gene expression alteration in LR. Conclusion It was inferred that PPAR-γ coupled signaling pathways maybe promote glycogen synthesis in forepart, prophase and anaphase of LR, cell proliferation and migration in the whole LR, while inhibits inflammation during LR.