基础医学与临床 ›› 2008, Vol. 28 ›› Issue (1): 61-65.

• 研究论文 • 上一篇    下一篇

卵圆细胞对肝损伤的修复作用及机制探讨

姚树坤 杨志云 常丽丽   

  1. 卫生部中日友好医院 河北医科大学 河北医科大学第四医院消化内科
  • 收稿日期:2006-12-13 修回日期:2007-03-27 出版日期:2008-01-25 发布日期:2008-01-25
  • 通讯作者: 姚树坤

Oval cells may play roles and its mechanism in the repair of hepatic injury induced by 2-AAF

Shu-kun YAO, Zhi-yun YANG, Li-li CHANG   

  1. China-Japan Friendship Hospital
  • Received:2006-12-13 Revised:2007-03-27 Online:2008-01-25 Published:2008-01-25
  • Contact: Shu-kun YAO,

摘要: 目的 探讨卵圆细胞在肝损伤中的修复作用,并对其可能的作用机制进行初步探讨。 方法 清结级SD大鼠50只随机分为正常对照组(20只)和模型组(30只),两组均于实验 第5周取肝组织进行常规病理、透射电镜, 免疫组化检测波形蛋白(vimentin)和增殖细胞核抗原(PCNA)蛋白表达, 半定量RT-PCR方法检测Notch1、RBP-Jκ、HES1 mRNA表达。 结果 正常对照组大鼠肝组织学形态正常,偶见vimentin 和PCNA阳性细胞,Notch1、RBP-Jκ、HES1 mRNA低表达;模型组主要于汇管区发现大量卵圆细胞,电镜下可见卵圆细胞的特征性表现张力微丝和桥粒连接,免疫组化示vimentin 和PCNA阳性细胞明显增加(P<0.01),Notch1、RBP-Jκ、HES1 mRNA表达显著上调(P<0.01)。结论 卵圆细胞在肝损伤修复中可能发挥重要作用,其作用机制与Notch家族有关。

Abstract: Objective To explore the role of oval cells in hepatic injure and to study its mechanism. Methods: Fifty male SD rats were randomly divided into normal control group(20 cases) and model group (30 cases). Hepatic pathologic changes were observed under optical microscope and electron microscope . Vimentin and PCNA were detected by immunohistochemistry, Notch1、 RBP-Jκ、HES1 mRNA were detected by semi-quantitative RT-PCR method. Results: The histological morphology was normal in the control group, the positive cells of vimentin and PCNA were found on occasion,the expression of Notch1、RBP-Jκ、HES1 mRNA was low.In the model group ,the oval cells were discovered in portal duct regions massively. The tonofilaments and desmosome juntion which were the distinctive structure of oval cells were observed by electron microscopy. The positive cells of vimentin and PCNA were increased (P<0.01), the expression of Notch1、RBP-Jκ、HES1 mRNA were increased (P<0.01). Conclusion: The oval cells play an important role in repair of hepatic injury ,the mechanism might relate to Notch family.