基础医学与临床 ›› 2012, Vol. 32 ›› Issue (8): 930-934.

• 研究论文 • 上一篇    下一篇

人脐带间充质干细胞抑制同种异体脐血淋巴细胞的转化

张玉琳   

  1. 宁波天一职业技术学院
  • 收稿日期:2011-09-27 修回日期:2011-12-02 出版日期:2012-08-05 发布日期:2012-07-17
  • 通讯作者: 张玉琳 E-mail:zhangyulin219@sohu.com

Umbilical Cord Mesenchymal Stem Cells inhibits the transformation of lymphocytes from allogeneic Umbilical Cord Blood

  • Received:2011-09-27 Revised:2011-12-02 Online:2012-08-05 Published:2012-07-17

摘要: 摘要:目的 探讨人脐带间充质干细胞(UCMSCs)的生物学特性及其对同种异体脐血淋巴细胞转化的影响。方法 从人脐带中分离、培养得到间充质干细胞,通过免疫细胞化学染色等方法对其进行表型鉴定;同时采用MTT法检测UCMSCs对同种异体脐血淋巴细胞转化的影响,以验证UCMSCs在免疫调控方面的作用。结果 成功培养出UCMSCs,培养至第3代的UBMSCs形态为均一梭形,细胞倍增时间为51.6h。细胞表面表达MSCs 相关抗原CD29,但不表达造血细胞相关抗原CD34,细胞化学染色显示PAS(+)、NBE(+)、POX(-),表明此种自脐带中分离得到并诱导扩增的成纤维样细胞为间充质干细胞;UCMSCs对PHA刺激的脐血淋巴细胞,增殖反应具有免疫抑制作用,且该作用与UCMSCs接种数量有关,当接种量为2 000个/孔时,抑制率高达60.8%。结论 UCMSCs具有免疫抑制作用,能抑制同种异体脐血淋巴细胞的转化,且此种抑制作用与接种的脐血淋巴细胞数量有关。

关键词: 关键词:脐带, 间充质干细胞, 免疫抑制

Abstract: Abstract: Objective To study the biological characteristics of mesenchymal stem cells(MSCs) derived from human cord(UCMSCs) and inhibitory effect on the transformation of lymphocytes from allogeneic Umbilical Cord Blood . Methods UCMSCs were isolated from umbilical cord ,the purity of MSC was analyzed by ICC.The effect of UCMSCs on the transformation of lymphocytes from allogeneic UCB was tested by the method of MTT. Results The morphological feature of the third passage’s UCMSCs(P3-UCMSCs) displayed an uniform fibroblast-like phenotype. The doubling time of P3-MSCs was 51.6 hours,they expressed MSCs-related antigen CD29 but did not express hematopoietic cell antigen CD34.The histochemical staining of P3-UCMSCs showed that PAS and NBE were positive but POX was negative. It indicated that the fibroblast-like cells induced and expanded from human cord in our experiment were MSCs ;UCB-lymphocyte transformation was suppressed when UCMSCs were cultured together with UCB-lymphocytes and the suppression effect was related with UCMSCs numbers plated in eath well, the suppression rate reached 60.8% when 2000 UCMSCs were plated. Conclusion UCMSCs had an immunomodulatory function. UCMSCs could suppress the transformation of lymphocytes from allogeneic UC and the suppression function seemed dependent on MSC numbers plated in each well.

Key words: Key Words: Umbilical cord, Mesenchymalstem cell, Immunomodulatory suppression