基础医学与临床 ›› 2021, Vol. 41 ›› Issue (8): 1121-1126.

• 研究论文 • 上一篇    下一篇

Wnt-5a/JNK信号通路参与PM2.5诱导的人支气管上皮细胞系16HBE分泌IL-6和TNF-α

邹威凤*, 王晓茜, 盛青, 祝涛, 周晓婷   

  1. 广州市胸科医院 呼吸科, 广东 广州 510095
  • 收稿日期:2020-11-27 修回日期:2021-04-07 出版日期:2021-08-05 发布日期:2021-07-21
  • 通讯作者: *szwei555@163.com
  • 基金资助:
    广东省自然科学基金(2020A1515010264);广州市科技计划项目(202002030080)

Wnt-5a/JNK is involved in PM2.5-induced expression of IL-6 and TNF-α of human bronchial epithelial cell line 16HBE

ZOU Wei-feng*, WANG Xiao-qian, SHENG Qing, ZHU Tao, ZHOU Xiao-ting   

  1. Department of Respiratory Medicine, Guangzhou Chest Hospital, Guangzhou 510095, China
  • Received:2020-11-27 Revised:2021-04-07 Online:2021-08-05 Published:2021-07-21
  • Contact: *szwei555@163.com

摘要: 目的 探讨PM2.5是否通过Wnt-5a/JNK信号通路诱导人支气管上皮细胞系16HBE中IL-6和TNF-α的分泌。方法 本研究分为大鼠和16HBE细胞两部分。具体如下:将大鼠分为对照组和吸入机动车尾气组(浓度1.5 mg/m2,2 h/d,1月);将16HBE细胞分为对照组、PM2.5组(20 μg/mL)、阴性对照siRNA+PM2.5组、Wnt-5a siRNA+PM2.5组、Wnt-5a siRNA+对照组、JNK抑制剂SP600125+PM2.5组、SP600125+对照组。采用免疫组化检测肺组织p-JNK和p-c-Jun表达水平;Western blot检测细胞中Wnt-5a、JNK、c-Jun、p-JNK和p-c-Jun表达水平; ELISA检测IL-6和TNF-α的分泌。结果 与大鼠对照组相比,机动车尾气组的p-JNK和p-c-Jun表达水平显著增加(P<0.05);与16HBE细胞对照组相比,PM2.5组的Wnt-5a、p-JNK、p-c-Jun、IL-6和TNF-α的表达水平显著增加(P<0.05);与PM2.5组相比,Wnt-5a siRNA+PM2.5组Wnt-5a、p-JNK、p-c-Jun、IL-6和TNF-α的表达水平显著减少(P<0.05),SP600125+PM2.5组p-c-Jun、IL-6和TNF-α的表达水平显著减少(P<0.05)。结论 Wnt-5a/JNK信号通路参与了PM2.5诱导的人支气管上皮细胞系分泌IL-6和TNF-α。

关键词: 人支气管上皮细胞系, PM2.5, Wnt-5a/JNK, IL-6, TNF-α

Abstract: Objective To explore whether PM2.5 induces the secretion of IL-6 and TNF-α in 16HBE via the Wnt-5a/JNK signaling pathway. Methods The research was implemented by rats and 16HBE cells. Rats were divided into control group and motor vehicle exhaust(MVE) group(concentration 1.5 mg/m2,2 h/day); 16HBE cells were divided into control group,PM2.5(20 μg/mL)group,control siRNA+PM2.5 group,Wnt-5a siRNA+PM2.5 group,Wnt-5a siRNA+control group,JNK inhibitor SP600125+PM2.5 group and SP600125+control group. The expressions of p-JNK and p-c-Jun in lung tissues were examined by immune-histochemistry method. The expressions of Wnt-5a,JNK,c-Jun,p-JNK and p-c-Jun were measured by Western blot. The secretions of IL-6 and TNF-α were assessed by ELISA. Results Compared with the control rat group, MVE group exhibited significantly up-regulated the expression of p-JNK and p-c-Jun(P<0.05); Compared with the 16HBE cell control group,PM2.5 group significantly up-regulated the expressions of Wnt-5a,p-JNK and p-c-Jun and the secretions of IL-6 and TNF-α(P<0.05);Compared with the PM2.5 group, Wnt-5a siRNA+PM2.5 group blocked these effects(P<0.05). Furthermore,SP600125+PM2.5 group showed a significantly decreased expression of p-c-Jun and the secretion of IL-6 and TNF-α by 16HBE cells(P<0.05). Conclusions PM2.5 induces the secretions of IL-6 and TNF-α by 16HBE cells via Wnt-5a/JNK signaling pathway.

Key words: 16 human bronchial epithelial cell line, PM2.5, Wnt-5a/JNK, IL-6, TNF-α

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