基础医学与临床 ›› 2020, Vol. 40 ›› Issue (9): 1212-1217.

• 研究论文 • 上一篇    下一篇

分泌型卷曲相关蛋白2抑制人结直肠癌细胞系SW480的增殖和迁移

周菁, 李索妮*   

  1. 陕西省肿瘤医院 肿瘤内科, 陕西 西安 710061
  • 收稿日期:2020-03-31 修回日期:2020-06-27 出版日期:2020-09-05 发布日期:2020-09-04
  • 通讯作者: *suonilishanxi@163.com
  • 基金资助:
    陕西省重点研发计划项目(2017SF-080)

Secreted frizzled-related protein 2 inhibits the proliferation and migration of human colorectal cancer cell line SW480

ZHOU Jing, LI Suo-ni*   

  1. Department of Oncology, Shaanxi Province Tumor Hospital, Xi'an 710061, China
  • Received:2020-03-31 Revised:2020-06-27 Online:2020-09-05 Published:2020-09-04
  • Contact: *suonilishanxi@163.com

摘要: 目的 探究分泌型卷曲相关蛋白2(SFRP2)抑制人结直肠癌细胞系SW480增殖、迁移的分子机制。方法 在人正常结肠上皮细胞HCoEpiC和结直肠癌细胞系SW480中采用Western blot和RT-qPCR检测SFRP2的表达;构建shSERP2稳转SW480细胞株;在shNC SW480和shSFRP2 SW480细胞中采用Western blot和RT-qPCR检测SFRP2、低氧诱导因子-1α(HIF-1α)和Twist的表达;HIF-1α抑制剂(IDF-11774)刺激shSFRP2 SW480细胞后,CCK-8法检测细胞增殖,Transwell小室法检测细胞迁移、侵袭。结果 SW480细胞中SFRP2的表达量低于HCoEpic细胞(P<0.01); 敲降SFRP2后,SW480细胞增殖、迁移、侵袭能力显著提高(P<0.001);而细胞内HIF-1α和Twist表达水平显著提高(P<0.01);HIF-1α抑制剂(IDF-11774)刺激后,其增殖、迁移、侵袭能力显著回降(P<0.001)。结论 SFRP2通过调控HIF-1α-Twist信号轴发挥其抑癌作用。

关键词: 结直肠癌, 分泌型卷曲相关蛋白2 (SFRP2), 低氧诱导因子-1α(HIF-1α), Twist

Abstract: Objective To explore the molecular mechanism by which secreted frizzy-related protein 2 (SFRP2) inhibits proliferation and migration of human colorectal cancer cell line SW480. Methods The expression of SFRP2 was detected by Western blot and RT-qPCR in human normal colonic epithelial cells HCoEpiC and colorectal cancer cell line SW480. The shSERP2 SW480 cell strain was constructed. Western blot and RT-qPCR were used to detect the expression of SFRP2, hypoxia inducible factor-1α (HIF-1α), and Twist in shNC SW480 and shSFRP2 SW480 cells. After shSFRP2 SW480 cells were stimulated by HIF-1α inhibitor (IDF-11774), CCK-8 assay detected cell proliferation and Transwell detected cell migration and invasion. Results The expression level of SFRP2 in SW480 cells was lower than that in HCoEpiC cells (P<0.01). After SFRP2 knockout, the proliferation, migration and invasion ability of SW480 cells were significantly improved (P<0.001). The expression of HIF-1α and Twist in SW480 cells was increased (P<0.01). After HIF-1α inhibitor (IDF-11774) treatment of shSFRP2 SW480 cells, compared with the untreated group, the proliferation, migration and invasion ability of shSFRP2 SW480 cells were significantly decreased (P<0.001). Conclusions SFRP2 exerts certain anticancer effects through regulation of the HIF-1α/Twist signaling axis.

Key words: colorectal cancer, secreted frizzy-related protein 2(SFRP2), hypoxia inducible factor-1α(HIF-1α), Twist

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