右美托咪啶上调sirtuin3减轻小鼠肾缺血-再灌注损伤

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (3): 374-379.

右美托咪啶上调sirtuin3减轻小鼠肾缺血-再灌注损伤

武月玲^*, 曲海霞, 王俊星

运城市中心医院/山西医科大学第八临床医学院麻醉科, 山西运城 044000

收稿日期:2019-04-16 修回日期:2019-12-02 出版日期:2020-03-05 发布日期:2020-03-02
通讯作者: ^*wuyueling2356@aliyun.com

Dexmedetomidine relieves ischaemia-reperfusion injury of kidney via up-regulation of sirtuin 3

WU Yue-ling^*, QU Hai-xia, WANG Jun-xing

Department of Anesthesiology, Yuncheng Central Hospital/the 8th Clinical Medical College of Shanxi Medical University, Yuncheng 044000, China

Received:2019-04-16 Revised:2019-12-02 Online:2020-03-05 Published:2020-03-02
Contact: ^*wuyueling2356@aliyun.com

摘要/Abstract

摘要： 目的研究右美托咪啶(DEX)在肾缺血-再灌注(I/R)损伤小鼠中的保护作用及其机制。方法将小鼠分为假手术(sham)组、siRNA+sham组、肾I/R组(常规法)、DEX+I/R组(腹腔注射25 μg/kg DEX)、siRNA+I/R组及siRNA+DEX+I/R组;siRNA+sham组、siRNA+I/R组、siRNA+DEX+I/R组术前3 d经尾静脉注射5×10⁹ TU/kg慢病毒-去乙酰化酶3(SIRT3)siRNA。生化分析仪测定肾功能;HE染色法观察肾组织形态学;Tunel染色法检测肾组织凋亡;Western blot检测SIRT3、亲环素D(Cyp D)和细胞色素C(Cyt C) 表达。结果 DEX可降低小鼠肾I/R后肾脏损伤,上调SIRT3表达并下调Cyt C及乙酰化Cyp D蛋白表达水平(P＜0.05)。抑制SIRT3后可减轻DEX对肾脏的保护作用(P＜0.05)。结论 DEX减轻肾I/R损伤的作用可能与上调SIRT3相关。

关键词: 急性肾损伤, 右美托咪啶, 线粒体损伤, 去乙酰化酶3

Abstract: Objective To study the protective effect and mechanism of dexmedetomidine (DEX) on renal ischemia-reperfusion (I/R) injury in mice. Methods The mice were divided into sham group, siRNA+sham group, renal I/R group (conventional method), DEX+I/R group (25 μg/kg DEX, intraperitoneal injection), siRNA+I/R group and siRNA+DEX+I/R group; siRNA+sham group, siRNA+I/R group and siRNA+DEX+I/R group were injected 5×10⁹ TU/kg lentivirus-sirtuin3 (SIRT3) siRNA through tail vein 3 days before surgery. Renal function was measured by biochemical analyzer, renal histomorphology was observed by HE staining, renal tissue apoptosis was detected by Tunel staining, and the expression of SIRT3, cyclophilin D (Cyp D) and cytochrome C (Cyt C) were detected by Western blot. Results DEX reduced the renal pathological damage after I/R, up-regulate the expression of SIRT3 and down-regulated the expression of Cyt C and acetylated Cyp D (P＜0.05). Inhibition of SIRT3 attenuated the protective effect of DEX on kidney. Conclusions The role of DEX in alleviating renal I/R injury may be related to the up-regulation of SIRT3.

Key words: acute kidney injury, dexmedetomidine, mitochondrial damage, sirtuin 3

中图分类号:

R965.3

武月玲, 曲海霞, 王俊星. 右美托咪啶上调sirtuin3减轻小鼠肾缺血-再灌注损伤[J]. 基础医学与临床, 2020, 40(3): 374-379.

WU Yue-ling, QU Hai-xia, WANG Jun-xing. Dexmedetomidine relieves ischaemia-reperfusion injury of kidney via up-regulation of sirtuin 3[J]. Basic & Clinical Medicine, 2020, 40(3): 374-379.

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