基础医学与临床 ›› 2020, Vol. 40 ›› Issue (1): 41-47.

• 研究论文 • 上一篇    下一篇

3种不同来源CD146阳性间充质干细胞亚群的生物学特性比较

王巧玲1, 张晓彤3, 徐衍2, 张乃月3, 朱榕嘉1*, 赵春华1*   

  1. 1.中国医学科学院基础医学研究所 北京协和医学院基础学院 组织工程中心, 北京 100005;
    2.北京大学人民医院 血液科,北京 100044;
    3.中国中医科学院 广安门医院 皮肤科,北京 100053
  • 收稿日期:2019-10-25 修回日期:2019-11-22 出版日期:2020-01-05 发布日期:2019-12-27
  • 通讯作者: *zrjia2006@126.com;zhaochunhua@vip.163.com
  • 基金资助:
    国家重点研发计划(2016YFA0101000,2016YFA0101003,2018YFA0109800);中国医学科学院医学与健康科技创新工程(2017-I2M-3-007);高等学校学科创新引智计划 (B18007)

Comparison of biological characteristics of CD146 positive subpopulations of mesenchymal stem cells derived from three different sources

WANG Qiao-ling1, ZHANG Xiao-tong3, XU Yan2, ZHANG Nai-yue3, ZHU Rong-jia1*, ZHAO Chun-hua1*   

  1. 1. Center of Excellence in Tissue Engineering, Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC, Beijing 100005;
    2. Department of Hematology, Peking Union Medical College Hospital,Beijing 100005;
    3. Department of Dermatology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China
  • Received:2019-10-25 Revised:2019-11-22 Online:2020-01-05 Published:2019-12-27
  • Contact: *zrjia2006@126. com;zhaochunhua@ vip.163.com

摘要: 目的 比较脐带间充质干细胞(UCMSCs)、脂肪间充质干细胞(AMSCs)以及骨髓间充质干细胞(BM-MSCs)中CD146+细胞亚群的生物学特性。方法 用磁珠分选法分选不同组织来源的间充质干细胞,获得高纯度CD146+亚群;用流式细胞计量术分析表型;透射电子显微镜观察细胞结构;成脂诱导分化后油红O染色;RT-qPCR检测成脂相关基因LPL、C/EBPα和PPARγ表达;成骨诱导分化后ALP染色,检测成骨相关基因ALP、OPN和RUNX2表达;检测细胞干性基因及血管生成相关基因表达;检测细胞体外成管能力。结果 3种组织来源CD146+ MSCs具有相似的形态,表达除CD106外相似的细胞表面标志分子。与其他两种细胞比较,CD146+AMSCs表达更高的干性基因OCT-4、SOX2和NANOG。在相同的诱导时间,CD146+ UCMSCs成脂和成骨能力较其他两种来源的CD146+MSCs弱。3种组织来源的CD146+ MSCs均可表达血管内皮相关刺激因子BFGF、VEGF、Ang-1和EGF,但CD146+BM-MSCs具有更强的体外成管能力。结论 3种不同来源的CD146+MSC具有不同的生物学特性,为进一步研究不同组织来源的间充质干细胞的特定应用提供了基础。

关键词: 间充质干细胞, CD146, 血管生成, 生物学特性

Abstract: Objective To characterize the biological features of CD146+ cells from umbilical cord mesenchymal stem cells(UCMSCs), adipose-derived mesenchymal stem cells(AMSCs) and bone marrow mesenchymal stem cells(BM-MSCs). Methods MSCs from three tissues were sorted by magnetically activated cell sorting(MACS) to obtain highly purified CD146+ subpopulations. The phenotypes and ultra-structures of these three MSC were analyzed by flow-cytometry and transmission electron microscopy. The differentiation abilities were tested by adipogenic and osteogenic inductions. The expression of genes relative to adipogenesis, osteogenesis, stemness or angiogenesis were determined by RT-qPCR. Results CD146+ cells derived from three kinds of tissues had similar ultra-structures and the ability of angiogenesis. CD146+ AMSCs expressed highest levels of the OCT-4, SOX2 and NANOG genes.Under the same induction conditions, CD146+ AMSCs showed higher potential for adipogenesis and CD146+BM-MSCs showed higher potential for osteogenesis.However, CD146+ UMSCs had little ability for adipogenesis and osteogenesis. All of CD146+ MSCs from 3 tissues expressed BFGF, VEGF, Ang-1 and EGF although CD146+ BM-MSCs had stronger ability of angiogenesis in vitro. Conclusions CD146+ MSCs derived from the 3 sources exhibit different biological characteristics.These results provide a useful biological basis for further studies relative to application of MSCs.

Key words: mesenchymal stem cells, CD146, angiogenesis, biological characteristics

中图分类号: