基础医学与临床 ›› 2019, Vol. 39 ›› Issue (8): 1136-1140.

• 研究论文 • 上一篇    下一篇

下调整合素连接激酶表达抑制鼻咽癌细胞系CNE1和5-8f的增殖

梁秀妮,赵丹芸,吴平安,曾敬贤   

  1. 香港大学深圳医院
  • 收稿日期:2019-03-27 修回日期:2019-06-25 出版日期:2019-08-05 发布日期:2019-07-16
  • 通讯作者: 吴平安 E-mail:wupa@hku-szh.org
  • 基金资助:
    《下调ILK表达对人鼻咽癌细胞的转移和EMT转化的影响及其作用机制》深圳市科技计划项目 (JCYJ20160429190356200);深圳市卫生与计划生育系统科研项目 (201607027)。

Down-regulating integrin-linked kinase expression inhibits the proliferation of human nasopharyngeal carcinoma cell lines CNE1 and 5-8f

  • Received:2019-03-27 Revised:2019-06-25 Online:2019-08-05 Published:2019-07-16

摘要: 目的 探讨整合素连接激酶(ILK)在鼻咽癌细胞系CNE1和5-8f中的表达水平。用下调ILK表达水平和使用ILK特异性小分子抑制剂方法,研究ILK表达对鼻咽癌细胞增殖的影响及意义。方法 用免疫组化法检测鼻咽癌组织和普通鼻咽组织中ILK的表达情况,用qRT-PCR和Western blot检测ILK在鼻咽癌细胞系CNE1和5-8f中RNA和蛋白表达水平,用MTT法检测对鼻咽癌细胞增殖的影响。结果 ILK的表达水平在NPC组织中呈阳性或弱阳性,普通鼻咽组织中呈阴性。在鼻咽癌细胞系CNE1和5-8f细胞中siILK能显著降低ILK的表达水平(P<0.05),siRNA下调ILK表达能抑制鼻咽癌细胞增殖能力(P<0.05),ILK特异性小分子抑制剂(Cdp22)能够显著抑制鼻咽癌细胞系增殖能力(P<0.05)。结论 下调ILK表达可抑制鼻咽癌细胞的增殖,为鼻咽癌患者提供新的治疗靶点。

关键词: 鼻咽癌, 细胞增殖, ILK(Integrin-linked kinase)

Abstract: Objective To investigate the expression levels of integrin-linked kinase in human nasopharyngeal carcinoma cell lines CNE1 and 5-8f. The effect of ILK expression on the proliferation of nasopharyngeal carcinoma cells. Methods IHC was used to exam ILK expression in NPC and normal nasopharyngeal epithelium. qRT-PCR and Western blot experiments were used to detect the RNA expression levels and protein expression level of ILK in nasopharyngeal carcinoma cell lines CNE1 and 5-8f..and measure the knock down efficiency of ILK Small interfering RNA. MTT assay was used to evaluate the cell proliferation of nasopharyngeal carcinoma cells. Data analysis was performed using SPSS 22.0 statistical software. The T-test was used for comparison between groups. P<0.05 was considered statistically significant. Results ILK expressed in NPC tissue but not normal nasopharyngeal epithelium. siILK significantly decreased ILK expression in nasopharyngeal carcinoma cell lines CNE1 and 5-8f, (P<0.05). Down-regulation of ILK expression by siRNA can inhibit the proliferation of nasopharyngeal carcinoma cells, ILK-specific small molecule inhibitor (Cdp22) inhibits the proliferation of nasopharyngeal carcinoma cells(P<0.05). Conclusion Down-regulating ILK expression inhibits the proliferation of nasopharyngeal carcinoma cells and can be used as a new target for treatments of patients with nasopharyngeal carcinoma (NPC).

Key words: nasopharyngeal carcinoma, Cell proliferation, integrin-linked kinase