基础医学与临床 ›› 2018, Vol. 38 ›› Issue (5): 600-603.

• 研究论文 • 上一篇    下一篇

食管癌患者外周血中miR-181b-5p表达水平下降

古丽尼格尔·雪合拉提1,王延蛟2,祖力皮喀尔·阿卜杜热合曼1,娜孜拉木·玉苏甫江1,斯坎德尔·白克力1   

  1. 1. 新疆医科大学基础医学院
    2. 新疆医科大学
  • 收稿日期:2017-11-23 修回日期:2018-01-26 出版日期:2018-05-05 发布日期:2018-04-28
  • 通讯作者: 斯坎德尔·白克力 E-mail:ikdbakri@163.com
  • 基金资助:
    维医异常黑胆质型消化系统肿瘤患者共性外周血miRNA标记物的研究

Expression of miR-181-5p in peripheral blood from patients with esophageal cancer is down-regulated

  • Received:2017-11-23 Revised:2018-01-26 Online:2018-05-05 Published:2018-04-28

摘要: 目的 探讨miR-181b-5p在食管癌患者外周血中表达水平的变化为食管癌患者的诊断和预后评估的临床意义。方法 收集34例食管癌患者和26名健康志愿者全血标本分为食管癌组和正常对照组,对白细胞总RNA的miRNA芯片结果进行分析,筛选单一miRNA候选指标并用RT-qPCR 法进行验证。结果 与正常对照组相比食管癌组miR-181b-5p表达水平下调。结论 miR-181b-5p在食管癌中表达下调,可能与食管癌的发生、发展相关。

关键词: 食管癌, 外周血, MicroRNA芯片, miR-181b-5p

Abstract: Objective To investigate the expression of miR-181b-5p in peripheral blood of patients with esophageal cancer and to provide guidance for the diagnosis and prognosis of esophageal cancer. Methods Blood samples were collected from 34 cases of esophageal cancer and 26 cases of healthy volunteers . The single candidate miRNA was selected by RNA miRNA chip screening analysis.Then the expression of miR-181b-5p in the serum of patients was detected by RT-qPCR .Results Compares with the normal group, the expression of miR-181b-5p was down-regulated in esophageal cancer group. Conclusion The expression of miR-181b-5p in esophageal cancer is down regulated, which may be related to the occurrence and development of esophageal cancer.

Key words: esophageal cancer, peripheral blood , microRNA chip, miR-181b-5p